Intern
Graduiertenkolleg 2157

Latest Publications

Characterization of a Human Respiratory Mucosa Model to Study Odorant Metabolism

Mérignac-Lacombe J, Kornbausch N, Sivarajan R, Boichot V, Berg K, Oberwinkler H, Saliba AE, Loos HM, Ehret Kasemo T, Scherzad A, Bodem J, Buettner A, Neiers F, Erhard F, Hackenberg S, Heydel JM, Steinke M
 

J Agric Food Chem. 2024 May 22. DOI: 10.1021/acs.jafc.4c00752

The release of host-derived antibodies bound to the variant surface glycoprotein (VSG) of Trypanosoma brucei cannot be explained by pH-dependent conformational changes of the VSG dimer

Link F, Borges A, Karo O, Jungblut M, Müller T, Meyer-Natus E, Krüger T, Sachs S, Jones NG, Morphew M, Sauer M, Stigloher C, McIntosh JR, Engstler M

Elife 2024 Apr 15. DOI: 10.7554/eLife.91194

Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes

Link F, Borges A, Karo O, Jungblut M, Müller T, Meyer-Natus E, Krüger T, Sachs S, Jones NG, Morphew M, Sauer M, Stigloher C, McIntosh JR, Engstler M

Elife. 2024 Apr 15;12:RP91194. DOI: 10.7554/eLife.91194

 

SARS-CoV-2 tropism to intestinal but not gastric epithelial cells is defined by limited ACE2 expression

Paužuolis M, Fatykhova D, Zühlke B, Schwecke T, Neyazi M, Samperio-Ventayol P, Aguilar C, Schlegel N, Dökel S, Ralser M, Hocke A, Krempl C, Bartfeld S

Stem Cell Reports. 2024 Apr. DOI: 10.1016/j.stemcr.2024.03.008

Organoids as a tool to study the impact of heterogeneity in gastrointestinal epithelium on host-pathogen interactions

Paužuolis M, Samperio Ventayol P, Neyazi M, Bartfeld S

Clin Exp Immunol. 2024 Jan 20:uxae002. DOI: 10.1093/cei/uxae002

gd T cell-mediated cytotoxicity against patient-derived healthy and cancer cervical organoids

Dong J, Holthaus D, Peters C, Koster S, Ehsani M, Quevedo-Olmos A, Berger H, Zarobkiewicz M, Mangler M, Gurumurthy RK, Hedemann N, Chumduri C, Kabelitz D, Meyer TF

Front Immunol. 2023 Nov 27;14:1281646. doi: 10.3389/fimmu.2023.1281646

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms

Reuter C, Hauf L, Imdahl F, Sen R, Vafadarnejad E, Fey P, Finger T, Jones NG, Walles H, Barquist L, Saliba AE, Groeber-Becker F, Engstler M.

Nat Commun. 2023 Nov 23;14(1):7660. doi:10.1038/s41467-023-43437-2

RNA recording in single bacterial cells using reprogrammed tracrRNAs

Jiao C, Reckstadt C, König F, Homberger C, Yu J, Vogel J, Westermann AJ, Sharma CM, Beisel CL

Nat Biotechnol. 2023 Aug;41(8):1107-1116. doi: 10.1038/s41587-022-01604-8

Intracellular lifestyle of Chlamydia trachomatis and host-pathogen interactions

Stelzner K, Vollmuth N, Rudel T.

Nat Rev Microbiol. 2023 Jul;21(7):448-462. doi: 10.1038/s41579-023-00860-y

The Intracellular Amastigote of Trypanosoma cruzi Maintains an Actively Beating Flagellum

Won MM, Krüger T, Engstler M, Burleigh BA.

mBio. 2023 Apr 25;14(2):e0355622. doi: 10.1128/mbio.03556-22

 

A primary cell-based in vitro model of the human small intestine reveals host olfactomedin 4 induction in response to Salmonella Typhimurium infection.

Däullary T, Imdahl F, Dietrich O, Hepp L, Krammer T, Fey C, Neuhaus W, Metzger M, Vogel J, Westermann AJ, Saliba AE, Zdzieblo D.

Gut Microbes. 2023 Jan-Dec;15(1):2186109. doi: 10.1080/19490976.2023.2186109.

MYC multimers shield stalled replication forks from RNA polymerase.

Solvie D, Baluapuri A, Uhl L, Fleischhauer D, Endres T, Papadopoulos D, Aziba A, Gaballa A, Mikicic I, Isaakova E, Giansanti C, Jansen J, Jungblut M, Klein T, Schülein-Völk C, Maric H, Doose S, Sauer M, Beli P, Rosenwald A, Dobbelstein M, Wolf E, Eilers M.

Nature. 2022 Dec;612(7938):148-155. doi: 10.1038/s41586-022-05469-4.

Fibroblastic reticular cells mitigate acute GvHD via MHCII-dependent maintenance of regulatory T cells

Shaikh H, Pezoldt J, Mokhtari Z, Gamboa Vargas J, Le DD, Peña Mosca J, Arellano Viera E, Kern MA, Graf C, Beyersdorf N, Lutz MB, Riedel A, Büttner-Herold M, Zernecke A, Einsele H, Saliba AE, Ludewig B, Huehn J, Beilhack A.

JCI Insight. 2022 Nov 22;7(22):e154250. doi: 10.1172/jci.insight.154250

Lymphatic migration of unconventional T cells promotes site-specific immunity in distinct lymph nodes

Ataide MA, Knöpper K, Cruz de Casas P, Ugur M, Eickhoff S, Zou M, Shaikh H, Trivedi A, Grafen A, Yang T, Prinz I, Ohlsen K, Gomez de Agüero M, Beilhack A, Huehn J, Gaya M, Saliba AE, Gasteiger G, Kastenmüller W

Immunity. 2022 Oct 11;55(10):1813-1828.e9. doi: 10.1016/j.immuni.2022.07.019

Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxis.

Aguilar C, Pauzuolis M, Pompaiah M, Vafadarnejad E, Arampatzi P, Fischer M, Narres D, Neyazi M, Kayisoglu Ö, Sell T, Blüthgen N, Morkel M, Wiegering A, Germer CT, Kircher S, Rosenwald A, Saliba AE, Bartfeld S.

Nat Commun. 2022 Oct 5;13(1):5878. doi: 10.1038/s41467-022-33165-4.

Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection.

Endres LM, Jungblut M, Divyapicigil M, Sauer M, Stigloher C, Christodoulides M, Kim BJ, Schubert-Unkmeir A.

Fluids Barriers CNS. 2022 Oct 26;19(1):81. doi: 10.1186/s12987-022-00379-z.

c-Myc plays a key role in IFN-γ-induced persistence of Chlamydia trachomatis.

Vollmuth N, Schlicker L, Guo Y, Hovhannisyan P, Janaki-Raman S, Kurmasheva N, Schmitz W, Schulze A, Stelzner K, Rajeeve K, Rudel T.

Elife. 2022 Sep 26;11:e76721. doi: 10.7554/eLife.76721.

In Silico Studies Reveal Peramivir and Zanamivir as an Optimal Drug Treatment Even If H7N9 Avian Type Influenza Virus Acquires Further Resistance.

Edita Sarukhanyan, Tipack Ayothyapattanam Shanmugam, Thomas Dandekar

Molecules. 2022 Sep; 27(18): 5920. Published online 2022 Sep 12. doi: 10.3390/molecules27185920

A defined anthocyanin mixture sourced from bilberry and black currant inhibits Measles virus and various herpesviruses.

Sivarajan R, Oberwinkler H, Roll V, König EM, Steinke M, Bodem J.

BMC Complement Med Ther. 2022 Jul 8;22(1):181. doi: 10.1186/s12906-022-03661-7.

Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer.

Thiele JC, Jungblut M, Helmerich DA, Tsukanov R, Chizhik A, Chizhik AI, Schnermann MJ, Sauer M, Nevskyi O, Enderlein J.

Sci Adv. 2022 Jun 10;8(23):eabo2506. doi: 10.1126/sciadv.abo2506.

Establishment of the SIS scaffold-based 3D model of human peritoneum for studying the dissemination of ovarian cancer.

Herbert SL, Fick A, Heydarian M, Metzger M, Wöckel A, Rudel T, Kozjak-Pavlovic V, Wulff C.

J Tissue Eng. 2022 Mar 23;13:20417314221088514. doi: 10.1177/20417314221088514.

Transient regulatory T-cell targeting triggers immune control of multiple myeloma and prevents disease progression

Dahlhoff J, Manz H, Steinfatt T, Delgado-Tascon J, Seebacher E, Schneider T, Wilnit A, Mokhtari Z, Tabares P, Böckle D, Rasche L, Martin Kortüm K, Lutz MB, Einsele H, Brandl A, Beilhack A.

Leukemia 2022 Mar;36(3):790-800. doi: 10.1038/s41375-021-01422-y

Tissue Models for Neisseria gonorrhoeae Research-From 2D to 3D

Heydarian M, Rühl E, Rawal R, Kozjak-Pavlovic V

Front Cell Infect Microbiol. 2022 Feb 11;12:840122. doi: 10.3389/fcimb.2022.840122 . eCollection 2022.

Modelling Chlamydia and HPV co-infection in patient-derived ectocervix organoids reveals distinct cellular reprogramming.

Koster S, Gurumurthy RK, Kumar N, Prakash PG, Dhanraj J, Bayer S, Berger H, Kurian SM, Drabkina M, Mollenkopf HJ, Goosmann C, Brinkmann V, Nagel Z, Mangler M, Meyer TF, Chumduri C.

Nat Commun. 2022 Feb 24;13(1):1030. doi: 10.1038/s41467-022-28569-1.

Complement activation induces excessive T cell cytotoxicity in severe COVID-19

Georg P, Astaburuaga-García R, Bonaguro L, Brumhard S, Michalick L, Lippert LJ, Kostevc T, Gäbel C, Schneider M, Streitz M, Demichev V, Gemünd I, Barone M, Tober-Lau P, Helbig ET, Hillus D, Petrov L, Stein J, Dey HP, Paclik D, Iwert C, Mülleder M, Aulakh SK, Djudjaj S, Bülow RD, Mei HE, Schulz AR, Thiel A, Hippenstiel S, Saliba AE, Eils R, Lehmann I, Mall MA, Stricker S, Röhmel J, Corman VM, Beule D, Wyler E, Landthaler M, Obermayer B, von Stillfried S, Boor P, Demir M, Wesselmann H, Suttorp N, Uhrig A, Müller-Redetzky H, Nattermann J, Kuebler WM, Meisel C, Ralser M, Schultze JL, Aschenbrenner AC, Thibeault C, Kurth F, Sander LE, Blüthgen N, Sawitzki B;PA-COVID-19 Study Group

Cell. 2022 Feb 3;185(3):493-512.e25. doi: 10.1016/j.cell.2021.12.040

Nanobody-mediated macromolecular crowding induces membrane fission and remodeling in the African trypanosome

Hempelmann A, Hartleb L, van Straaten M, Hashemi H, Zeelen JP, Bongers K, Papavasiliou FN, Engstler M, Stebbins CE, Jones NG

Cell Rep. 2021 Nov 2;37(5):109923. doi: 10.1016/j.celrep.2021.109923

The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids

Borges AR, Link F, Engstler M, Jones NG.

Front Cell Dev Biol. 2021 Nov 1;9:720536. DOI: 10.3389/fcell.2021.720536

SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosis

Wendisch D, Dietrich O, Mari T, von Stillfried S, Ibarra IL, Mittermaier M, Mache C, Chua RL, Knoll R, Timm S, Brumhard S, Krammer T, Zauber H, Hiller AL, Pascual-Reguant A, Mothes R, Bülow RD, Schulze J, Leipold AM, Djudjaj S, Erhard F, Geffers R, Pott F, Kazmierski J, Radke J, Pergantis P, Baßler K, Conrad C, Aschenbrenner AC, Sawitzki B, Landthaler M, Wyler E, Horst D; Deutsche COVID-19 OMICS Initiative (DeCOI); Hippenstiel S, Hocke A, Heppner FL, Uhrig A, Garcia C, Machleidt F, Herold S, Elezkurtaj S, Thibeault C, Witzenrath M, Cochain C, Suttorp N, Drosten C, Goffinet C, Kurth F, Schultze JL, Radbruch H, Ochs M, Eils R, Müller-Redetzky H, Hauser AE, Luecken MD, Theis FJ, Conrad C, Wolff T, Boor P, Selbach M, Saliba AE, Sander LE.

Cell. 2021 Dec 22;184(26):6243-6261.e27. DOI: 10.1016/j.cell.2021.11.033

Susceptibility of Human Airway Tissue Models Derived From Different Anatomical Sites to Bordetella pertussis and Its Virulence Factor Adenylate Cyclase Toxin

Sivarajan R, Kessie DK, Oberwinkler H, Pallmann N, Walles T, Scherzad A, Hackenberg S, Steinke M.

Front Cell Infect Microbiol. 2021 Dec 23;11:797491. DOI: 10.3389/fcimb.2021.797491

PACmn for improved optogenetic control of intracellular cAMP

Yang S, Constantin OM, Sachidanandan D, Hofmann H, Kunz TC, Kozjak-Pavlovic V, Oertner TG, Nagel G, Kittel RJ, Gee CE, Gao S

BMC Biol. 2021 Oct 18;19(1):227. doi: 10.1186/s12915-021-01151-9

Staphylococcusaureus Transcriptome Data and Metabolic Modelling Investigate the Interplay of Ser/Thr Kinase PknB, Its Phosphatase Stp, the glmR/yvcK Regulon and the cdaA Operon for Metabolic Adaptation

Liang C, Rios-Miguel AB, Jarick M, Neurgaonkar P, Girard M, François P, Schrenzel J, Ibrahim ES, Ohlsen K, Dandekar T

Microorganisms. 2021 Oct 14;9(10):2148. doi: 10.3390/microorganisms9102148

The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei

Bakari-Soale M, Ikenga NJ, Scheibe M, Butter F, Jones NG, Kramer S, Engstler M.

Sci Rep. 2021 Sep 15;11(1):18325. doi:10.1038/s41598-021-97020-0

To the Surface and Back: Exo- and Endocytic Pathways in Trypanosoma brucei

Link F, Borges AR, Jones NG, Engstler M.

Front Cell Dev Biol. 2021 Aug 6:9:720521.

Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract

Shaikh H, Vargas JG, Mokhtari Z, Jarick KJ, Ulbrich M, Mosca JP, Viera EA, Graf C, Le DD, Heinze KG, Büttner-Herold M, Rosenwald A, Pezoldt J, Huehn J, Beilhack A

 

Front Immunol. 2021 Jul 26:12:689896. DOI: 10.3389/fimmu.2021.689896

 

 

Gastrointestinal epithelial innate immunity-regionalization and organoids as new model

Kayisoglu Ö, Schlegel N, Bartfeld S

J Mol Med (Berl). 2021 Apr;99(4):517-530. doi: 10.1007/s00109-021-02043-9. Epub 2021 Feb 4.

In vitro skin culture media influence the viability and inflammatory response of primary macrophages

The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy

Kunz TC, Rühling M, Moldovan A, Paprotka K, Kozjak-Pavlovic V, Rudel T, Fraunholz M

Front Cell Infect Microbiol. 2021 Mar 16;11:644750. doi: 10.3389/fcimb.2021.644750

Single-cell motile behaviour of [Formula: see text] in thin-layered fluid collectives

Krüger T, Maus K, Kreß V, Meyer-Natus E, Engstler M.

Eur Phys J E Soft Matter. 2021 Mar 23;44(3):37. DOI: 10.1140/epje/s10189-021-00052-7

Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia

Chumduri C, Gurumurthy RK, Berger H, Dietrich O, Kumar N, Koster S, Brinkmann V, Hoffmann K, Drabkina M, Arampatzi P, Son D, Klemm U, Mollenkopf HJ, Herbst H, Mangler M, Vogel J, Saliba AE, Meyer TF.

Nat Cell Biol. 2021 Feb;23(2):184-197. DOI: 10.1038/s41556-020-00619-0

 

 

Activity of Tracheal Cytotoxin of Bordetella pertussis in a Human Tracheobronchial 3D Tissue Model

Kessie DK, Lodes N, Oberwinkler H, Goldman WE, Walles T, Steinke M, Gross R.

Front Cell Infect Microbiol. 2021 Jan 19;10:614994. DOI: 10.3389/fcimb.2020.614994