Orthogonal approaches are needed to identify the full targetome of a bacterial sRNA
16.02.2021By complementing previous target screens with RNA affinity purification, a formerly overlooked target of the virulence-related Salmonella sRNA PinT is uncovered.
PinT-mediated repression of the translation of the secreted virulence factor SteC affects host actin rearrangement in infected cells. Thus, this sRNA helps Salmonella time its virulence expression along the infection cycle. This new study was led by the Würzburg laboratories of Jörg Vogel and Alexander Westermann.
Publication: Sara Correia Santos, Thorsten Bischler, Alexander J. Westermann, Jörg Vogel: MAPS integrates regulation of actin-targeting effector SteC into the virulence control network of Salmonella small RNA PinT. Cell Reports. 2021 Feb 2;34(5):108722. doi:10.1016/j.celrep.2021.108722.
