Publikationen

2020

Kappenberger J, Koelmel W, Schoenwetter E, Scheuer T, Woerner, J, Kuper J*., Kisker C*. How to limit the speed of a motor: The intricate regulation oft he XPB ATPase and translocase in TFIIH. Nucleic Acids Res., 2020 Nov 16;gkaa911.doi: 10.1093/nar/gkaa911. Online ahead of print.

Barnett JT, Kuper J, Koelmel W, Kisker C*, Kad NM*. The TFIIH subunits p44/p62 act as a damage sensor during nucleotide excision repair. Nucleic Acids Res., 2020 Nov 9;gkaa973.doi: 10.1093/nar/gkaa973. Online ahead of print.

Peissert S, Schlosser A, Kendel R, Kuper J*, Kisker C*. Structural basis for CDK7 activation by MAT1 and Cyclin H, Proc Natl Acad Sci USA first published October 14, 2020. PMID: 33055219 doi: 10.1073/pnas.2010885117.

Volz J, Kusch C, Beck S, Popp M, Vögtle T, Meub M, Scheller I, Heil HS, Preu J, Schuhmann MK, Hemmen K, Premsler T, Sickmann A, Heinze KG, Stegner D, Stoll G, Braun A, Sauer M, Nieswandt B. BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation. J Clin Invest. 2020 Oct 12:136457. doi: 10.1172/JCI136457. Online ahead of print.PMID: 32750041

Anna KL Liess, Alena Kucerova, Kristian Schweimer, Dörte Schlesinger, Olexandr Dybkov, Henning Urlaub, Jörg Mansfeld, and Sonja Lorenz Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S. Sci Signal. 2020 Oct 20;13(654):eaba8208. doi: 10.1126/scisignal.aba8208.

Kasaragod, V. B., Pacios-Michelena, A., Schaefer, N., Zheng, F., Bader, N., Alzheimer, C., Villmann, C., Schindelin, H. Pyridoxal Kinase Inhibition by Artemisinins Downregulates Inhibitory Neurotransmission. bioRxiv, 2020.2004.2005.026310, doi:10.1101/2020.04.05.026310 (2020).

Green, J. L., Wu, Y., Encheva, V., Lasonder, E., Prommaban, A., Kunzelmann, S., Christodoulou, E., Grainger, M., Truongvan, N., Bothe, S., Sharma, V., Song, W., Pinzuti, I., Uthaipibull, C., Srichairatanakool, S., Birault, V., Langsley, G., Schindelin, H., Stieglitz, B., Snijders, A. P., Holder, A. A. Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development. PLoS Pathog 16, e1008640, doi:10.1371/journal.ppat.1008640 (2020).

Jeanclos, E., Knobloch, G., Hoffmann, A., Fedorchenko, O., Odersky, A., Lamprecht, A. K., Schindelin, H., Gohla, A. Ca(2+) functions as a molecular switch that controls the mutually exclusive complex formation of pyridoxal phosphatase with CIB1 or calmodulin. FEBS Lett, doi:10.1002/1873-3468.13795 (2020).

Dietschreit, J. C. B., Wagner, A., Le, T. A., Klein, P., Schindelin, H., Opatz, T., Engels, B., Hellmich, U. A., Ochsenfeld, C. Predicting (19) F NMR Chemical Shifts: A Combined Computational and Experimental Study of a Trypanosomal Oxidoreductase-Inhibitor Complex. Angew Chem Int Ed Engl 59, 12669-12673, doi:10.1002/anie.202000539 (2020).

Mostosi, P., Schindelin, H., Kollmannsberger, P. & Thorn, A. Haruspex: A Neural Network for the Automatic Identification of Oligonucleotides and Protein Secondary Structure in Cryo-Electron Microscopy Maps. Angew Chem Int Ed Engl 59, 14788-14795, doi:10.1002/anie.202000421 (2020).

Bangalore DM, Heil HS, Mehringer CF, Hirsch L, Hemmen K, Heinze KG, Tessmer I. Automated AFM analysis of DNA bending reveals initial lesion sensing strategies of DNA glycosylases.  Sci Rep. 2020 Sep 23;10(1):15484. doi: 10.1038/s41598-020-72102-7.PMID: 32968112 Free PMC article.

Gerovac M, El Mouali Y, Kuper J, Kisker C, Barquist L, Vogel J. Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein. RNA. 2020 Jul 9:rna.076992.120. doi: 10.1261/rna.076992.120. Epub ahead of print. PMID: 32646969.

Gorelashvili MG, Angay O, Hemmen K, Klaus V, Stegner D, Heinze KG. Megakaryocyte volume modulates bone marrow niche properties and cell migration dynamics. Haematologica. 2020 Apr;105(4):895-904. doi: 10.3324/haematol.2018.202010. Epub 2019 Jun 27.PMID: 31248970

Scheller I, Stritt S, Beck S, Peng B, Pleines I, Heinze KG, Braun A, Otto O, Ahrends R, Sickmann A, Bender M, Nieswandt B. Coactosin-like 1 integrates signaling critical for shear-dependent thrombus formation in mouse platelets.  Haematologica. 2020 Jun;105(6):1667-1676. doi: 10.3324/haematol.2019.225516. Epub 2019 Oct 3.PMID: 31582545 Free PMC article.

Schuhmann MK, Kraft P, Bieber M, Kollikowski AM, Schulze H, Nieswandt B, Pham M, Stegner D, Stoll G. Targeting Platelet GPVI Plus rt-PA Administration but Not 21-Mediated Collagen Binding Protects against Ischemic Brain Damage in Mice. Int J Mol Sci. 2019 Apr 24;20(8):2019. doi: 10.3390/ijms20082019.

Peissert S, Sauer F, Grabarczyk DB, Braun C, Sander G, Poterszman A, Egly JM, Kuper J, Kisker C. In TFIIH the Arch domain of XPD is mechanistically essential for transcription and DNA repair. Nat Commun. 2020 Apr 3;11(1):1667. doi: 10.1038/s41467-020-15241-9.

David Stegner, Katrin G Heinze: Intravital imaging of megakaryocytes. Platelets. 2020 Mar 10:1-11. doi: 10.1080/09537104.2020.1738366. Online ahead of print.

Amich J, Mokhtari Z, Strobel M, Vialetto E, Sheta D, Yu Y, Hartweg J, Kalleda N, Jarick KJ, Brede C, Jordán-Garrote AL, Thusek S, Schmiedgen K, Arslan B, Pinnecker J, Thornton CR, Gunzer M, Krappmann S, Einsele H, Heinze KG*, Beilhack A*: Three-Dimensional Light Sheet Fluorescence Microscopy of Lungs To Dissect Local Host Immune-Aspergillus fumigatus Interactions. mBio. 2020 Feb 4;11(1):e02752-19. doi: 10.1128/mBio.02752-19. *corresponding author

Ries LK, Liess AKL, Feiler CG, Spratt DE, Lowe ED, Lorenz S. (2020). Crystal structure of the catalytic C-lobe of the HECT-type ubiquitin ligase E6AP. Protein Sci. 2020 doi: 10.1002/pro.3832. [Epub ahead of print]

Langlhofer G, Schaefer N, Maric HM, Keramidas A, Zhang Y, Baumann P, Blum R, Breitinger U, Strømgaard K, Schlosser A, Kessels MM, Koch D, Qualmann B, Breitinger HG, Lynch JW, Villmann C. "A novel glycine receptor variant with startle disease affects syndapin I and glycinergic inhibition." Journal of Neuroscience. 30 April 2020, doi: 10.1523/JNEUROSCI.2490-19.2020

Beckwitt EC, Jang S, Carnaval Detweiler I, Kuper J, Sauer F, Simon N, Bretzler J, Watkins SC, Carell T, Kisker C, Van Houten B. Single molecule analysis reveals monomeric XPA bends DNA and undergoes episodic linear diffusion during damage search. Nat Commun. 2020 Apr 3;11(1):1667. doi: 10.1038/s41467-020-15241-9.

Rill N, Mukhortava A, Lorenz S, Tessmer I. Alkyltransferase-like protein clusters scan DNA rapidly over long distances and recruit NER to alkyl-DNA lesions. Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9318-9328. doi: 10.1073/pnas.1916860117. Epub 2020 Apr 9. PMID: 32273391

Horvat S, Y Yu, S Böjte, I Tessmer, DW Lowman, Z Ma, DL Williams, A Beilhack, K Albrecht, J Groll (2020) Engineering nanogels for Drug Delivery to Pathogenic Fungi Aspergillus fumigatus by Tuning Polymer Amphiphilicity, Biomacromolecules, epub ahead of print

Onwubiko NO, A Borst, SA Diaz, K Passkowski, F Scheffel, I Tessmer*, HP Nasheuer* (2020) SV40 T antigen interactions with ssDNA and replication protein A: a regulatory role of T antigen monomers in lagging strand DNA replication, Nucleic Acids Research 48(7): 3657-3677 (* joint correspondence)

Prieto-Garcia C, Hartmann O, Reissland M, Braun F, Fischer T, Walz S, Schülein-Völk C, Eilers U, Ade CP, Calzado MA, Orian A, Maric HM,  Münch C, Rosenfeldt M, Eilers M, Diefenbacher ME. Maintaining protein stability of ∆Np63 via USP28 is required by squamous cancer cells. EMBO Mol Med. 2020 Mar 4;:e11101. doi: 10.15252/emmm.201911101.

Špiranec Spes K, Hupp S, Werner F, Koch F, Völker K, Krebes L, Kämmerer U, Heinze KG, Braunger BM, Kuhn M. Natriuretic Peptides Attenuate Retinal Pathological Neovascularization Via Cyclic Guanosine Monophosphate Signaling in Pericytes and Astrocytes. Arterioscler Thromb Vasc Biol. 2020 Jan;40(1):159-174. doi: 10.1161/ATVBAHA.119.313400. Epub 2019 Oct 17.PMID: 31619060 

Langlhofer, G.; Schaefer, N.; Maric, H. M.; Keramidas, A.; Zhang, Y.; Baumann, P.; Blum, R.; Breitinger, U.; Strømgaard, K.; Schlosser, A.; Kessels, M. M.; Koch, D.; Qualmann, B.; Breitinger, H.-G.; Lynch, J. W.; Villmann, C. A Novel Glycine Receptor Variant with Startle Disease Affects Syndapin I and Glycinergic Inhibition. J. Neurosci. 2020, 40 (25), 4954–4969. doi.org/10.1523/JNEUROSCI.2490-19.2020.

Hör, J.; Garriss, G.; Di Giorgio, S.; Hack, L.-M.; Vanselow, J. T.; Förstner, K. U.; Schlosser, A.; Henriques‐Normark, B.; Vogel, J. Grad‐seq in a Gram‐positive Bacterium Reveals Exonucleolytic sRNA Activation in Competence Control. EMBO J. 2020, 39 (9). doi.org/10.15252/embj.2019103852.

Kraus, A. J.; Vanselow, J. T.; Lamer, S.; Brink, B. G.; Schlosser, A.; Siegel, T. N. Distinct Roles for H4 and H2A.Z Acetylation in RNA Transcription in African Trypanosomes. Nat. Commun. 2020, 11 (1), 1498. doi.org/10.1038/s41467-020-15274-0.

Ost, G. S.; Wirth, C.; Bogdanović, X.; Kao, W.-C.; Schorch, B.; Aktories, P. J. K.; Papatheodorou, P.; Schwan, C.; Schlosser, A.; Jank, T.; Hunte, C.; Aktories, K. Inverse Control of Rab Proteins by Yersinia ADP-Ribosyltransferase and Glycosyltransferase Related to Clostridial Glucosylating Toxins. Sci. Adv. 2020, 6 (11), eaaz2094. doi.org/10.1126/sciadv.aaz2094.

Adhikari, B.; Bozilovic, J.; Diebold, M.; Schwarz, J. D.; Hofstetter, J.; Schröder, M.; Wanior, M.; Narain, A.; Vogt, M.; Dudvarski Stankovic, N.; Baluapuri, A.; Schönemann, L.; Eing, L.; Bhandare, P.; Kuster, B.; Schlosser, A.; Heinzlmeir, S.; Sotriffer, C.; Knapp, S.; Wolf, E. PROTAC-Mediated Degradation Reveals a Non-Catalytic Function of AURORA-A Kinase. Nat. Chem. Biol. 2020. doi.org/10.1038/s41589-020-00652-y.

Schreiner, P.; Harrer, T.; Scheibenbogen, C.; Lamer, S.; Schlosser, A.; Naviaux, R. K.; Prusty, B. K. Human Herpesvirus-6 Reactivation, Mitochondrial Fragmentation, and the Coordination of Antiviral and Metabolic Phenotypes in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome. ImmunoHorizons 2020, 4 (4), 201–215. doi.org/10.4049/immunohorizons.2000006.

Erhard, F.; Dölken, L.; Schilling, B.; Schlosser, A. Identification of the Cryptic HLA-I Immunopeptidome. Cancer Immunol. Res. 2020, canimm.0886.2019. doi.org/10.1158/2326-6066.CIR-19-0886.

 

 

 

2019

Schurr Y, Sperr A, Volz J, Beck S, Reil L, Kusch C, Eiring P, Bryson S, Sauer M, Nieswandt B, Machesky L, Bender M. Platelet lamellipodium formation is not required for thrombus formation and stability. Blood Volume: 134 Issue: 25 Pages: 2318-2329 Dec 19 2019.

Geue S, Aurbach K, Manke MC, Manukjan G, Münzer P, Stegner D, Brähler C, Walker-Allgaier B, Märklin M, Borst CE, Quintanilla-Fend L, Rath D, Geisler T, Salih HR, Seizer P, Lang F, Nieswandt B, Gawaz M, Schulze H, Pleines I, Borst O. Pivotal role of PDK1 in megakaryocyte cytoskeletal dynamics and polarization during platelet biogenesis. Blood Volume: 134 Issue: 21 Pages: 1847-1858 ‏Nov 21 2019.

Kasaragod, V. B. & Schindelin, H. Structure of Heteropentameric GABA_A Receptors and Receptor-Anchoring Properties of Gephyrin. Front Mol Neurosci 12, 191, doi:10.3389/fnmol.2019.00191 (2019).

Hänzelmann, P., Galgenmüller, C. & Schindelin, H. Structure and Function of the AAA+ ATPase p97, a Key Player in Protein Homeostasis. Subcell Biochem 93, 221-272, doi:10.1007/978-3-030-28151-9_7 (2019).

Kitzenmaier, A., Schaefer, N., Kasaragod, V. B., Polster, T., Hantschmann, R., Schindelin, H., Villmann, C. The P429L loss of function mutation of the human glycine transporter 2 associated with hyperekplexia. Eur J Neurosci 50, 3906-3920, doi:10.1111/ejn.14533 (2019).

Viet, K. K., Wagner, A., Schwickert, K., Hellwig, N., Brennich, M., Bader, N., Schirmeister, T., Morgner, N., Schindelin, H., Hellmich, U. A. Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure 27, 1246-1257.e1245, doi:10.1016/j.str.2019.04.016 (2019).

Bansal, A., Karanth, N. M., Demeler, B., Schindelin, H. & Sarma, S. P. Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases. Biochemistry 58, 1992-2008, doi:10.1021/acs.biochem.9b00050 (2019).

Stegner D, Klaus V, Nieswandt B. Platelets as Modulators of Cerebral Ischemia/Reperfusion Injury. Frontiers in Immunology Volume: 10 Article Number: 2505 Nov 1 2019. Review.

Architecture of the mycobacterial type VII secretion system. Famelis N., Rivera-Calzada A., Degliesposti G., Wingender M., Mietrach N., Skehel J. M., Fernandez-Leiro R., Böttcher B., Schlosser A., Llorca O. and Geibel S. (2019). Nature Oct 9; doi: 10.1038/s41586-019-1633-1.

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes. Grimm C., Hillen H. S., Bedenk K., Bartuli J., Neyer S., Zhang Q., Huttenhofer A., Erlacher M., Dienemann C., Schlosser A., Urlaub H., Böttcher B., Szalay A. A., Cramer P. and Fischer U. (2019). Cell Dec 12; 179 (7): 1537-50 e19. doi: 10.1016/j.cell.2019.11.024.

Wollbrink A., Dobel C., Salvari V., Paraskevopoulos E., Kisker C., Mittmann K., Pantev C. Choice of test stimulus matters for pitch matching performance: Comparison between pure tone and narrow band noise. Hear Res. 2019 Sep 15;381:107776. doi: 10.1016/j.heares.2019.107776.

Homologous bd oxidases share the same architecture but differ in mechanism. Thesseling# A., Rasmussen# T., Burschel S., Wohlwend D., Kagi J., Müller R., Böttcher* B. and Friedrich* T. (2019). Nat Commun Nov 13; 10 (1): 5138. doi: 10.1038/s41467-019-13122-4.

Stegner D, Hofmann S, Schuhmann MK, Kraft P, Herrmann AM, Popp S, Höhn M, Popp M, Klaus V, Post A, Kleinschnitz C, Braun A, Meuth SG, Lesch KP, Stoll G, Kraft R, Nieswandt B. Loss of Orai2-Mediated Capacitative Ca2+ Entry Is Neuroprotective in Acute Ischemic Stroke Stroke. 2019 Nov; 50(11):3238-3245. doi: 10.1161/STROKEAHA.119.025357. Epub 2019 Sep 25.

Noy PJ, Gavin RL, Colombo D, Haining EJ, Reyat JS, Payne H, Thielmann I, Lokman AB, Neag G, Yang J, Lloyd T, Harrison N, Heath VL, Gardiner C, Whitworth KM, Robinson J, Koo CZ, Di Maio A, Harrison P, Lee SP, Michelangeli F, Kalia N, Rainger GE, Nieswandt B, Brill A, Watson SP, Tomlinson MG. Tspan18 is a novel regulator of the Ca2+ channel Orai1 and von Willebrand factor release in endothelial cells. Haematologica. 2019 Sep; 104(9):1892-1905. doi: 10.3324/haematol.2018.194241. Epub 2018 Dec 20.

Structure of the Mechanosensitive Channel MscS Embedded in the Membrane Bilayer. Rasmussen T., Flegler V. J., Rasmussen A. and Böttcher B. (2019). J Mol Biol Aug 9; 431 (17): 3081-90. doi: 10.1016/j.jmb.2019.07.006.

Rieckmann M, Delgobo M, Gaal C, Büchner L, Steinau P, Reshef D, Gil-Cruz C, Horst ENT, Kircher M, Reiter T, Heinze KG, Niessen HW, Krijnen PA, van der Laan AM, Piek JJ, Koch C, Wester HJ, Lapa C, Bauer WR, Ludewig B, Friedman N, Frantz S, Hofmann U, Ramos GC. Myocardial infarction triggers cardioprotective antigen-specific T helper cell responses.  J Clin Invest. 2019 Aug 13;129(11):4922-4936. doi: 10.1172/JCI123859.PMID: 31408441 Free PMC article

Nagy M, van Geffen JP, Stegner D, Adams DJ, Braun A, de Witt SM, Elvers M, Geer MJ, Kuijpers MJE, Kunzelmann K, Mori J, Oury C, Pircher J, Pleines I, Poole AW, Senis YA, Verdoold R, Weber C, Nieswandt B, Heemskerk JWM, Baaten CCFMJ. Comparative Analysis of Microfluidics Thrombus Formation in Multiple Genetically Modified Mice: Link to Thrombosis and Hemostasis. Front Cardiovasc Med. 2019 Jul 30; 6:99. doi: 10.3389/fcvm.2019.00099. eCollection 2019.

Stoll G, Nieswandt B. Thrombo-inflammation in acute ischaemic stroke - implications for treatment. Nat Rev Neurol. 2019 Aug; 15(8):473-481. doi: 10.1038/s41582-019-0221-1. Epub 2019 Jul 1.

Volz J, Mammadova-Bach E, Gil-Pulido J, Nandigama R, Remer K, Sorokin L, Zernecke A, Abrams SI, Ergün S, Henke E, Nieswandt B. Inhibition of platelet GPVI induces intratumor hemorrhage and increases efficacy of chemotherapy in mice. Blood. 2019 Jun 20; 133(25):2696-2706. doi: 10.1182/blood.2018877043. Epub 2019 Apr 5.

Kiran Gotru S, van Geffen JP, Nagy M, Mammadova-Bach E, Eilenberger J, Volz J, Manukjan G, Schulze H, Wagner L, Eber S, Schambeck C, Deppermann C, Brouns S, Nurden P, Greinacher A, Sachs U, Nieswandt B, Hermanns HM, Heemskerk JWM, Braun A. Defective Zn2+ homeostasis in mouse and human platelets with alpha- and delta-storage pool diseases. Sci Rep. 2019 Jun 6; 9(1):8333. doi: 10.1038/s41598-019-44751-w.

Capabilities of the Falcon III detector for single-particle structure determination. Song, B., J. Lenhart, V. J. Flegler, C. Makbul, T. Rasmussen and B. Böttcher (2019). Ultramicroscopy 203: 145-154.

Liess A, Kucerova A, Schweimer K, Yu L, Roumeliotis T, Diebold M, Dybkov O, Sotriffer C, Urlaub H, Choudhary J, Mansfeld J, Lorenz S (2019). Auto-inhibition mechanism of the ubiquitin-conjugating enzyme UBE2S by auto-ubiquitination. Structure 27(8):1195-1210

featured in Bodrug T, Brown NG (2019). UBE2S learns self control. Structure 27(8):11185-1187 

Deol KK, Lorenz S*, Strieter ER (2019). Enzymatic Logic of Ubiquitin Chain Assembly. Front Physiol. 10:835

Ries LK, Sander B, Deol KK, Letzelter MA, Strieter ER, Lorenz S (2019). Analysis of ubiquitin recognition by the HECT ligase E6AP provides insight into its linkage specificity. J Biol Chem 294: 6113-6129

Sereikaite, V., T. Fritzius, V. Kasaragod, N. Bader, H. M. Maric, H. Schindelin, B. Bettler and K. Strømgaard (2019). Targeting the γ-aminobutyric acid type B (GABAB) receptor complex: Development of inhibitors targeting the K+ channel tetramerization domain (KCTD) containing proteins/GABAB receptor protein-protein interaction. Journal of Medicinal Chemistry. doi: 10.1021/acs.jmedchem.9b01087. [Epub ahead of print]

Khayenko, V., and Maric, H.M. (2019). Targeting GABAAR-Associated Proteins: New Modulators, Labels and Concepts. Frontiers in Molecular Neuroscience 12. doi: 10.3389/fnmol.2019.00162

Lee HJ, Li CF, Ruan D, He J, Montal ED, Lorenz S, Girnun GD, Chan CH. Non-proteolytic ubiquitination of Hexokinase 2 by HectH9 controls tumor metabolism and cancer stem cell expansion. Nat Commun. 2019 Jun 14;10(1):2625. doi: 10.1038/s41467-019-10374-y. PMID: 31201299

Sdelci, S., Rendeiro, A.F., Rathert, P., You, W., Lin, J.-M.G., Ringler, A., Hofstätter, G., Moll, H.P., Gürtl, B., Farlik, M., Schick, S., Klepsch, F., Oldach, M., Buphamalai, P., Schischlik, F., Májek, P., Parapatics, K., Schmidl, C., Schuster, M., Penz, T., Buckley, D.L., Hudecz, O., Imre, R., Wang, S.-Y., Maric, H.M., Kralovics, R., Bennett, K.L., Müller, A.C., Mechtler, K., Menche, J., Bradner, J.E., Winter, G.E., Klavins, K., Casanova, E., Bock, C., Zuber, J., and Kubicek, S. (2019). MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nature Genetics.  doi: 10.1038/s41588-019-0413-z

Baluapuri A, Hofstetter J, Dudvarski Stankovic N, Endres T, Bhandare P, Vos SM, Adhikari B Schwarz JD, Narain A, Vogt M, Wang SJ, Düster R, Jung LA, Vanselow JT, Wiegering A, Geyer M, Maric HM, Gallant P, Walz S, Schlosser A, Cramer P, Eilers M, Wolf E. MYC recruits SPT5 to RNA polymerase II to promote processive transcription elongation. Molecular Cell (2019) doi.org/10.1016/j.molcel.2019.02.031

Neidler S, Kruspig B, Hewit K, Monteverde T, Gyuraszova K, Braun A, Clark W, James D, Hedley A, Nieswandt B, Shanks E, Dick C, Murphy DJ. Identification of a Clinically Relevant Signature for Early Progression in KRAS-Driven Lung Adenocarcinoma. Cancers (Basel). 2019 Apr 29;11(5):600. doi: 10.3390/cancers11050600.

Schuhmann MK, Kraft P, Bieber M, Kollikowski AM, Schulze H, Nieswandt B, Pham M, Stegner D, Stoll G. Targeting Platelet GPVI Plus rt-PA Administration but Not 21-Mediated Collagen Binding Protects against Ischemic Brain Damage in Mice. Int J Mol Sci. 2019 Apr 24;20(8):2019. doi: 10.3390/ijms20082019.

Kruber P, Angay O, Winkler A, Bösl MR, Kneitz S, Heinze KG, Gessler M. Loss or oncogenic mutation of DROSHA impairs kidney development and function, but is not sufficient for Wilms tumor formation. Int J Cancer. 2019 Mar 15;144(6):1391-1400. doi: 10.1002/ijc.31952. Epub 2018 Dec 3.PMID: 30367465

Sauer F, Klemm T, Kollampally RB, Tessmer I, Nair RK, Popov N, Kisker C. Differential Oligomerization of the Deubiquitinases USP25 and USP28 Regulates Their Activities. Mol Cell. 2019 May 2;74(3):421-435.e10. doi: 10.1016/j.molcel.2019.02.029. Epub 2019 Mar 26

Malehmir M, Pfister D, Gallage S, Szydlowska M, Inverso D, Kotsiliti E, Leone V, Peiseler M, Surewaard BGJ, Rath D, Ali A, Wolf MJ, Drescher H, Healy ME, Dauch D, Kroy D, Krenkel O, Kohlhepp M, Engleitner T, Olkus A, Sijmonsma T, Volz J, Deppermann C, Stegner D, Helbling P, Nombela-Arrieta C, Rafiei A, Hinterleitner M, Rall M, Baku F, Borst O, Wilson CL, Leslie J, O'Connor T, Weston CJ, Adams DH, Sheriff L, Teijeiro A, Prinz M, Bogeska R, Anstee N, Bongers MN, Notohamiprodjo M, Geisler T, Withers DJ, Ware J, Mann DA, Augustin HG, Vegiopoulos A, Milsom MD, Rose AJ, Lalor PF, Llovet JM, Pinyol R, Tacke F, Rad R, Matter M, Djouder N, Kubes P, Knolle PA, Unger K, Zender L, Nieswandt B, Gawaz M, Weber A, Heikenwalder M. Platelet GPIbα is a mediator and potential interventional target for NASH and subsequent liver cancer.  Nat Med. 2019 Apr; 25(4):641-655. doi: 10.1038/s41591-019-0379-5. Epub 2019 Apr 1.

Nasir N, Kisker C. Mechanistic insights into the enzymatic activity and inhibition of the replicative polymerase exonuclease domain from Mycobacterium tuberculosis. DNA Repair (Amst). 2019 Feb;74:17-25. doi: 10.1016/j.dnarep.2018.12.006.

Kasaragod, V., Hausrat, T.J., Schaefer, N., Kuhn, M., Christensen, N.R., Tessmer, I., Maric, H.M., Lindegaard Madsen, K., Sotriffer, C., Villmann, C., Kneussel, M., Schindelin, H. Elucidating the Molecular Basis for Inhibitory Neurotransmission Regulation by Artemisinins. Neuron 2019 Jan 28  doi:doi.org/10.1016/j.neuron.2019.01.001

Bieber M, Schuhmann MK, Volz J, Kumar GJ, Vaidya JR, Nieswandt B, Pham M, Stoll G, Kleinschnitz C, Kraft P. Description of a Novel Phosphodiesterase (PDE)-3 Inhibitor Protecting Mice From Ischemic Stroke Independent From Platelet Function. Stroke. 2019 Feb; 50(2):478-486. doi: 10.1161/STROKEAHA.118.023664.

Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA. Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl. 2019 Jan 3. doi: 10.1002/anie.201810470. [Epub ahead of print]

Mostosi, P., Schindelin, H., Kollmannsberger, P., Thorn*, A. (2019) Automated interpretation of Cryo-EM density maps with convolutional neural networks. DOI: 10.1101/644476 [preprint]

Ries LK, Sander B, Deol KK, Letzelter MA, Strieter ER, Lorenz S. Analysis of ubiquitin recognition by the HECT ligase E6AP provides insight into its linkage specificity. Biol Chem. 2019 Apr 12;294(15):6113-6129. doi: 10.1074/jbc.RA118.007014. Epub 2019 Feb 8. PMID: 30737286

Rayes J, Watson SP, Nieswandt B. Functional significance of the platelet immune receptors GPVI and CLEC-2. J Clin Invest. 2019 Jan 2; 129(1):12-23. doi: 10.1172/JCI122955. Epub 2019 Jan 2.

Mammadova-Bach E, Nagy M, Heemskerk JWM, Nieswandt B, Braun A. Store-operated calcium entry in thrombosis and thrombo-inflammation. Cell Calcium. 2019 Jan; 77:39-48. doi: 10.1016/j.ceca.2018.11.005. Epub 2018 Nov 23.

Albert-Weissenberger C, Hopp S, Nieswandt B, Sirén AL, Kleinschnitz C, Stetter C. How is the formation of microthrombi after traumatic brain injury linked to inflammation? J Neuroimmunol. 2019 Jan 15; 326:9-13. doi: 10.1016/j.jneuroim.2018.10.011. Epub 2018 Oct 31.
Review

Marcel Seibold; Thorsten Stühmer; Nadine Kremer; Anja Mottok; Claus-Jürgen Scholz; Andreas Schlosser; Ellen Leich; Ulrike Holzgrabe; Daniela Brünnert; Santiago Barrio; K. Martin Kortüm; Antonio G. Solimando; Manik Chatterjee; Hermann Einsele; Andreas Rosenwald; Ralf C. Bargou; Torsten Steinbrunn. RAL GTPases Mediate Multiple Myeloma Cell Survival and Are Activated Independently of Oncogenic RAS. Haematologica 2019, 105 (9), 2316–2326. doi.org/10.3324/haematol.2019.223024.

Mietrach, N.; Schlosser, A.; Geibel, S. An Extracellular Domain of the EsaA Membrane Component of the Type VIIb Secretion System: Expression, Purification and Crystallization. Acta Crystallogr. Sect. F Struct. Biol. Commun. 2019, 75 (12), 725–730. doi.org/10.1107/S2053230X1901495X.

Ortiz-Soto, M. E.; Reising, S.; Schlosser, A.; Seibel, J. Structural and Functional Role of Disulphide Bonds and Substrate Binding Residues of the Human Beta-Galactoside Alpha-2,3-Sialyltransferase 1 (HST3Gal1). Sci. Rep. 2019, 9 (1), 17993. doi.org/10.1038/s41598-019-54384-8.

Seibold, M.; Stühmer, T.; Mottok, A.; Scholz, C.; Chatterjee, M.; Leich, E.; Barrio, S.; Kortüm, K. M.; Schlosser, A.; Rosenwald, A.; Einsele, H.; Bargou, R. C.; Steinbrunn, T. Functional Characterization of the RAL-Dependent Survival Pathway in Multiple Myeloma. Clin. Lymphoma Myeloma Leuk. 2019, 19 (10), e138–e139. doi.org/10.1016/j.clml.2019.09.230.

El-Mesery, M.; Rosenthal, T.; Rauert-Wunderlich, H.; Schreder, M.; Stühmer, T.; Leich, E.; Schlosser, A.; Ehrenschwender, M.; Wajant, H.; Siegmund, D. The NEDD8-Activating Enzyme Inhibitor MLN4924 Sensitizes a TNFR1+ Subgroup of Multiple Myeloma Cells for TNF-Induced Cell Death. Cell Death Dis. 2019, 10 (8), 611. doi.org/10.1038/s41419-019-1860-2.

Pauls, D.; Hamarat, Y.; Trufasu, L.; Schendzielorz, T. M.; Gramlich, G.; Kahnt, J.; Vanselow, J. T.; Schlosser, A.; Wegener, C. Drosophila Carboxypeptidase D ( SILVER ) Is a Key Enzyme in Neuropeptide Processing Required to Maintain Locomotor Activity Levels and Survival Rate. Eur. J. Neurosci. 2019, 50 (9), 3502–3519. doi.org/10.1111/ejn.14516.

Baluapuri, A.; Hofstetter, J.; Dudvarski Stankovic, N.; Endres, T.; Bhandare, P.; Vos, S. M.; Adhikari, B.; Schwarz, J. D.; Narain, A.; Vogt, M.; Wang, S.-Y.; Düster, R.; Jung, L. A.; Vanselow, J. T.; Wiegering, A.; Geyer, M.; Maric, H. M.; Gallant, P.; Walz, S.; Schlosser, A.; Cramer, P.; Eilers, M.; Wolf, E. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation. Mol. Cell 2019, 74 (4), 674-687.e11. doi.org/10.1016/j.molcel.2019.02.031.

Bathon, K.; Weigand, I.; Vanselow, J. T.; Ronchi, C. L.; Sbiera, S.; Schlosser, A.; Fassnacht, M.; Calebiro, D. Alterations in Protein Kinase A Substrate Specificity as a Potential Cause of Cushing Syndrome. Endocrinology 2019, 160 (2), 447–459. doi.org/10.1210/en.2018-00775. 

Li, Y. H.; Liu, X.; Vanselow, J. T.; Zheng, H.; Schlosser, A.; Chiu, J. C. O-GlcNAcylation of PERIOD Regulates Its Interaction with CLOCK and Timing of Circadian Transcriptional Repression. PLOS Genet. 2019, 15 (1), e1007953. doi.org/10.1371/journal.pgen.1007953.

Levanova, N.; Steinemann, M.; Böhmer, K. E.; Schneider, S.; Belyi, Y.; Schlosser, A.; Aktories, K.; Jank, T. Characterization of the Glucosyltransferase Activity of Legionella Pneumophila Effector SetA. Naunyn. Schmiedebergs. Arch. Pharmacol. 2019, 392 (1), 69–79. doi.org/10.1007/s00210-018-1562-9.

 

 

2018

Hannah S. Heil, Benjamin Schreiber, Ralph Götz, Monika Emmerling, Marie-Christine Dabauvalle, Georg Krohne, Sven Hoefling, Martin Kamp, Markus Sauer, Katrin G. Heinze: Sharpening emitter localization in front of a tuned mirror; Light: Science and Applications; December 2018; DOI: https://doi.org/10.1038/s41377-018-0104-z

Hepatitis B virus core protein phosphorylation: Identification of the SRPK1 target sites and impact of their occupancy on RNA binding and capsid structure. Heger-Stevic J., Zimmermann P., Lecoq L., Böttcher B. and Nassal M. (2018). PLoS Pathog Dec 19; 14 (12): e1007488. doi: 10.1371/journal.ppat.1007488.

Structure of Mutant Hepatitis B Core Protein Capsids with Premature Secretion Phenotype.Böttcher B. and Nassal M. (2018). J Mol Biol 430 4941–54. doi: 10.1016/j.jmb.2018.10.018.

Geometrical and Structural Dynamics of Imatinib within Biorelevant Colloids. Wiest J., Saedtler M., Böttcher B., Grune M., Reggane M., Galli B., Holzgrabe U. and Meinel L. (2018). Mol Pharm Aug 28; 15 4470-80. doi: 10.1021/acs.molpharmaceut.8b00469.

Schreiber, Benjamin; Kauk, Michael; Heil, Hannah; Emmerling, Monika; Tessmer, Ingrid; Kamp, Martin; Höfling, Sven; Holzgrabe, Ulrike; Hoffmann, Carsten; Heinze, Katrin: Enhanced fluorescence resonance energy transfer in G protein-coupled receptor probes by nano-coated microscopy coverslips; ACS Photonics, March 2018, DOI: 10.1021/acsphotonics.8b0007

Giroud M., Dietzel U., Anselm L., Banner D., Kuglstatter A., Benz J., Blanc J.B., Gaufreteau D., Liu H., Lin X., Stich A., Kuhn B., Schuler F., Kaiser M., Brun R., Schirmeister T., Kisker C., Diederich F., Haap W. (2018). Repurposing a Library of Human Cathepsin L Ligands: Identification of Macrocyclic Lactams as Potent Rhodesain and Trypanosoma brucei Inhibitors. J Med Chem. 2018 Apr 26;61(8):3350-3369. doi: 10.1021/acs.jmedchem.7b01869. Epub 2018 Apr 13.

Chen D, Gehringer M, Lorenz S (2018) Developing small-molecule inhibitors of HECT-type ubiquitin ligases for therapeutic applications: challenges and opportunities. ChemBioChem 19(20):2123-2135

Hines, R., Maric, H.M., Hines, D., Modgil, A., Panzanelli, P., Nakamura, Y., Nathanson, A., Cross, A., Deeb, T., Brandon, N., Davies, P., Fritschy, J.M., Schindelin, H., Moss, S. (2018) Developmental seizures and mortality result from reducing GABA_A Receptor α2 subunit interaction with collybistin. Nature Communications, 9, 3130 (2018) doi: 10.1038/s41467-018-05481-1

Bleem, A., Christiansen, G., Madsen, D.J., Maric, H.M., Strømgaard, K., Bryers, J.D., Daggett, V., Meyer, R.L., Otzen, D.E., (2018) Protein Engineering Reveals Mechanisms of Functional Amyloid Formation in Pseudomonas aeruginosa Biofilms, Journal of Molecular Biology (2018) doi.org/10.1016/j.jmb.2018.06.043

Büchel G., Carstensen A., Mak K.Y., Roeschert I., Leen E., Sumara O., Hofstetter J., Herold S., Kalb J., Baluapuri A., Poon W, Kwok C, Chesler L, Maric H.M., Rickman D.S., Wolf E., Bayliss R., Walz S., Eilers M. Association with Aurora-A Controls N-MYC-Dependent Promoter Escape and Pause Release of RNA Polymerase II during the Cell Cycle. Cell Reports, Volume 21 , Issue 12 , 3483 – 3497 (2018) doi  10.1016/j.celrep.2017.11.090

Manukjan G, Eilenberger J, Andres O, Schambeck C, Eber S, Schulze H. Functional classification of pediatric patients with non-syndromic delta-storage pool deficiency. Hämostaseologie. 2018, manuscript in press.

Aurbach K, Spindler M, Haining EJ, Bender M, Pleines I. Blood collection, platelet isolation and measurement of platelet count and size in mice – a practical guide. Platelets. 2018, manuscript in press.

Mammadova-Bach E, Mauler M, Braun A, Duerschmied D. Autocrine and paracrine regulatory functions of platelet serotonin. Platelets. 2018 Sep;29(6):541-548. doi: 10.1080/09537104.2018.1478072.

Gorelashvili MG, Heinze KG, Stegner D. Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy. Methods Mol Biol. 2018;1812:233-253. doi: 10.1007/978-1-4939-8585-2_14.

Mekala SR, Wörsdörfer R, Bauer J, Stoll O, Wagner N, Reeh L, Loew K, Eckner G, Kwok CK, Wischmeyer E, Dickinson ME, Schulze H, Stegner D, Benndorf RA, Edenhofer F, Pfeiffer V, Kuerten S, Frantz S, Ergün S. Generation of Cardiomyocytes From Vascular Adventitia-Resident Stem Cells. Circ Res. 2018;123:686-99.

Bender M, Otto O. No Escape-A Novel Tool to Quantify Platelet Intra-Thrombus Movements. Thromb Haemost. 2018 Aug 15. doi: 10.1055/s-0038-1668587.

Spindler M, van Eeuwijk JMM, Schurr Y, Nurden P, Nieswandt B, Stegner D, Reinhold A, Bender M. ADAP deficiency impairs megakaryocyte polarization with ectopic proplatelet release and causes microthrombocytopenia. Blood. 2018 Aug 9;132(6):635-646. doi: 10.1182/blood-2018-01-829259.

Pleines I, Lebois M, Gangatirkar P, Au AE, Lane RM, Henley KJ, Kauppi M, Corbin J, Cannon P, Bernardini J, Alwis I, Jarman KE, Ellis S, Metcalf D, Jackson SP, Schoenwaelder SM, Kile BT, Josefsson EC. Intrinsic apoptosis circumvents the functional decline of circulating platelets but does not cause the storage lesion. Blood. 2018 Jul 12;132(2):197-209. doi: 10.1182/blood-2017-11-816355.

Pleines I, Cherpokova D, Bender M. Rho GTPases and their downstream effectors in megakaryocyte biology. Platelets. 2018 Jun 18:1-8. doi: 10.1080/09537104.2018.1478071.

Schulze H, Stegner D. Imaging platelet biogenesis in vivo. Res Pract Thromb Haemost. 2018 Jun 10;2(3):461-468. doi: 10.1002/rth2.12112.

Andres O, Henning K, Strauß G, Pflug A, Manukjan G, Schulze H. Diagnosis of platelet function disorders: A standardized, rational, and modular flow cytometric approach. Platelets. 2018 Jun;29(4):347-356. doi: 10.1080/09537104.2017.1386297.

Cao H, Biondo M, Lioe H, Busfield S, Rayzman V, Nieswandt B, Bork K, Harrison LC, Auyeung P, Farkas H, Csuka D, Pelzing M, Dower S, Wilson MJ, Nash A, Nolte MW, Panousis C. Antibody-mediated inhibition of FXIIa blocks downstream bradykinin generation. J Allergy Clin Immunol. 2018 Jun 21. pii: S0091-6749(18)30901-1. doi: 10.1016/j.jaci.2018.06.014.

Giles JA, Greenhalgh AD, Denes A, Nieswandt B, Coutts G, McColl BW, Allan SM.  Neutrophil infiltration to the brain is platelet-dependent, and is reversed by blockade of platelet GPIbα. Immunology. 2018 Jun;154(2):322-328. doi: 10.1111/imm.12892.

Stritt S, Birkholz I, Beck S, Sorrentino S, Sapra KT, Viaud J, Heck J, Gaits-Iacovoni F, Schulze H, Du X, Hartwig JH, Braun A, Bender M, Medalia O, Nieswandt B. Profilin 1-mediated cytoskeletal rearrangements regulate integrin function in mouse platelets. Blood Adv. 2018 May 8;2(9):1040-1045. doi:10.1182/bloodadvances.2017014001.

Pircher J, Czermak T, Ehrlich A, Eberle C, Gaitzsch E, Margraf A, Grommes J, Saha P, Titova A, Ishikawa-Ankerhold H, Stark K, Petzold T, Stocker T, Weckbach LT, Novotny J, Sperandio M, Nieswandt B, Smith A, Mannell H, Walzog B, Horst D, Soehnlein O, Massberg S, Schulz C. Cathelicidins prime platelets to mediate arterial thrombosis and tissue inflammation. Nat Commun. 2018 Apr 18;9(1):1523. doi: 10.1038/s41467-018-03925-2.

Gotru SK, Gil-Pulido J, Beyersdorf N, Diefenbach A, Becker IC, Vögtle T, Remer K, Chubanov V, Gudermann T, Hermanns HM, Nieswandt B, Kerkau T, Zernecke A, Braun A. Cutting Edge: Imbalanced Cation Homeostasis in MAGT1-Deficient B Cells Dysregulates B Cell Development and Signaling in Mice. J Immunol. 2018 Apr 15;200(8):2529-2534. doi: 10.4049/jimmunol.1701467.

Nagy M, Mastenbroek TG, Mattheij NJA, de Witt S, Clemetson KJ, Kirschner J, Schulz AS, Vraetz T, Speckmann C, Braun A, Cosemans JMEM, Zieger B, Heemskerk JWM. Variable impairment of platelet functions in patients with severe, genetically linked immune deficiencies. Haematologica. 2018 Mar;103(3):540-549. doi: 10.3324/haematol.2017.176974.

Gupta S, Cherpokova D, Spindler M, Morowski M, Bender M, Nieswandt B. GPVI signaling is compromised in newly formed platelets after acute thrombocytopenia in mice. Blood. 2018 Mar 8;131(10):1106-1110. doi: 10.1182/blood-2017-08-800136.

Rayes J, Jadoui S, Lax S, Gros A, Wichaiyo S, Ollivier V, Denis CV, Ware J, Nieswandt B, Jandrot-Perrus M, Watson SP, Ho-Tin-Noé B. The contribution of platelet glycoprotein receptors to inflammatory bleeding prevention is stimulus and organ dependent. Haematologica. 2018 Jun;103(6):e256-e258. doi: 10.3324/haematol.2017.182162. Epub 2018 Feb 1.

Claushuis TAM, de Vos AF, Nieswandt B, Boon L, Roelofs JJTH, de Boer OJ, van  't Veer C, van der Poll T. Platelet glycoprotein VI aids in local immunity during pneumonia-derived sepsis caused by gram-negative bacteria. Blood. 2018 Feb 22;131(8):864-876. doi: 10.1182/blood-2017-06-788067.

Gotru SK, Chen W, Kraft P, Becker IC, Wolf K, Stritt S, Zierler S, Hermanns HM, Rao D, Perraud AL, Schmitz C, Zahedi RP, Noy PJ, Tomlinson MG, Dandekar T, Matsushita M, Chubanov V, Gudermann T, Stoll G, Nieswandt B, Braun A. TRPM7 Kinase Controls Calcium Responses in Arterial Thrombosis and Stroke in Mice. Arterioscler Thromb Vasc Biol. 2018 Feb;38(2):344-352. doi: 10.1161/ATVBAHA.117.310391.

Cloutier N, Allaeys I, Marcoux G, Machlus KR, Mailhot B, Zufferey A, Levesque T, Becker Y, Tessandier N, Melki I, Zhi H, Poirier G, Rondina MT, Italiano JE, Flamand L, McKenzie SE, Cote F, Nieswandt B, Khan WI, Flick MJ, Newman PJ, Lacroix S, Fortin PR, Boilard E. Platelets release pathogenic serotonin and return to circulation after immune complex-mediated sequestration. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1550-E1559. doi: 10.1073/pnas.1720553115.

Schuhmann MK, Kraft P, Bieber M, Haarmann A, Homola GA, Pham M, Nieswandt B, Stoll G. Influence of Thrombolysis on the Safety and Efficacy of Blocking Platelet Adhesion or Secretory Activity in Acute Ischemic Stroke in Mice. Transl Stroke Res. 2018 Jan 10. doi: 10.1007/s12975-017-0606-7.

Hines RM, Maric HM, Hines DJ, Modgil A, Panzanelli P, Nakamura Y, Nathanson AJ, Cross A, Deeb T, Brandon NJ, Davies P, Fritschy JM, Schindelin H, Moss SJ. Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin. Nat Commun. 2018 Aug 7;9(1):3130. doi: 10.1038/s41467-018-05481-1.

Turakhiya A, Meyer SR, Marincola G, Böhm S, Vanselow JT, Schlosser A, Hofmann K and Buchberger A (2018). ZFAND1 recruits p97 and the 26S proteasome to promote the clearance of arsenite-induced stress granules. Mol Cell 70:906-919.

Gholamreza Fazeli, Maurice Stetter, Jaime N. Lisack und Ann M. Wehman (2018): C. elegans blastomeres clear the corpse of the second polar body by LC3-associated phagocytosis; Cell Report; DOI: 10.1016/j.celrep.2018.04.043

Beer, K.B.,  Rivas-Castillo, J., Kuhn, K., Fazeli, G., Karmann, B., Nance, J.F., Stigloher, C. and Wehman A.M. (2018) Extracellular vesicle budding is inhibited by redundant regulators of TAT-5 flippase localization and phospholipid asymmetry. PNAS. DOI:10.1073/pnas.1714085115

 

 

2017

Near-Atomic Resolution Structure of a Plant Geminivirus Determined by Electron Cryomicroscopy. Hipp K., Grimm C., Jeske H. and Böttcher B. (2017). Structure Aug 01; 25 (8): 1303-9 e3. doi: 10.1016/j.str.2017.06.013.

Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Sundaramoorthy R., Hughes A. L., Singh V., Wiechens N., Ryan D. P., El-Mkami H., Petoukhov M., Svergun D. I., Treutlein B., Quack S., Fischer M., Michaelis J., Böttcher B., Norman D. G. and Owen-Hughes T. (2017). Elife Mar 23; 6 doi: 10.7554/eLife.22510.

Ingold I, Berndt C, Schmitt S, Doll S, Poschmann G, Buday K, Roveri A, Peng X, Porto Freitas F, Seibt T, Mehr L, Aichler M, Walch A, Lamp D, Jastroch M, Miyamoto S, Wurst W, Ursini F, Arnér ESJ, Fradejas-Villar N, Schweizer U, Zischka H, Friedmann Angeli JP, Conrad M. Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis. Cell. 2017 Dec 16. pii: S0092-8674(17)31438-1. doi: 10.1016/j.cell.2017.11.048.

Stockwell BR, Friedmann Angeli JP, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascón S, Hatzios SK, Kagan VE, Noel K, Jiang X, Linkermann A, Murphy ME, Overholtzer M, Oyagi A, Pagnussat GC, Park J, Ran Q, Rosenfeld CS, Salnikow K, Tang D, Torti FM, Torti SV, Toyokuni S, Woerpel KA, Zhang DD. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. Cell. 2017 Oct 5;171(2):273-285. doi: 10.1016/j.cell.2017.09.021

Angeli JPF, Shah R, Pratt DA, Conrad M. Ferroptosis Inhibition: Mechanisms and Opportunities.Trends Pharmacol Sci. 2017 May;38(5):489-498. doi: 10.1016/j.tips.2017.02.005

Zilka O, Shah R, Li B, Friedmann Angeli JP, Griesser M, Conrad M, Pratt DA. On the Mechanism of Cytoprotection by Ferrostatin-1 and Liproxstatin-1 and the Role of Lipid Peroxidation in Ferroptotic Cell Death. ACS Cent Sci. 2017 Mar 22;3(3):232-243. doi: 10.1021/acscentsci.7b00028.

Doll S, Proneth B, Tyurina YY, Panzilius E, Kobayashi S, Ingold I, Irmler M, Beckers J, Aichler M, Walch A, Prokisch H, Trümbach D, Mao G, Qu F, Bayir H, Füllekrug J, Scheel CH, Wurst W, Schick JA, Kagan VE, Angeli JP*, Conrad M*. ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition. Nat Chem Biol. 2017 Jan;13(1):91-98. doi: 10.1038/nchembio.2239.

Kagan VE, Mao G*, Qu F*, Angeli JP*, Doll S, Croix CS, Dar HH, Liu B, Tyurin VA, Ritov VB, Kapralov AA, Amoscato AA, Jiang J, Anthonymuthu T, Mohammadyani D, Yang Q, Proneth B, Klein-Seetharaman J, Watkins S, Bahar I, Greenberger J, Mallampalli RK, Stockwell BR, Tyurina YY, Conrad M, Bayır H. Oxidized arachidonic and adrenic PEs navigate cells to ferroptosis. Nat Chem Biol. 2017 Jan;13(1):81-90. doi: 10.1038/nchembio.2238.

Stegner D, vanEeuwijk JMM, Angay O, Gorelashvili MG, Semeniak D, Pinnecker J, Schmithausen P, Meyer I, Friedrich M, Dütting S, Brede C, Beilhack A, Schulze H, Nieswandt B, Heinze KG. Thrombopoiesis is spatially regulated by the bone marrow vasculature. Nat Commun. 2017 Jul 25;8(1):127. doi: 10.1038/s41467-017-00201-7.

Grabarczyk, D.B., Silkenat S., Kisker C. (2018). Structural Basis fort he Recruitment of Ctf18-RFC to the Replisome. Structure 2018 Jan 2;26(1):137-144.e3. doi: 10.1016/j.str.2017.11.004. Epub 2017 Dec 7.

Skinner J.J., Wang S., Lee J., Ong C., Sommese R., Sivaramakrishnan S., Koelmel W., Hirschbeck M., Schindelin H., Kisker C., Lorenz K., Sosnick T.R., Rosner M.R. (2017). Conserved salt-bridge competition triggered by phosphorylation regulates the protein interactome. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):13453-13458. doi: 10.1073/pnas.1711543114. Epub 2017 Dec 5.

Radu L., Schoenwetter E., Braun C., Marcoux J., Koelmel W., Schmitt D.R., Kuper J., Cianférani S., Egly J.M., Poterszman A., Kisker C. (2017). The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH. Nucleic Acids Res. 2017 Oct 13;45(18):10872-10883. doi: 10.1093/nar/gkx743.

Bartossek T., Jones N.G., Schäfer C., Cvitković M., Glogger M., Mott H.R., Kuper J., Brennich M., Carrington M., Smith A.S., Fenz S., Kisker C., Engstler M. (2017). Structural basis for the shielding function of the dynamic trypanosome variant surface glycoprotein coat. Nat Microbiol. 2017 Nov;2(11):1523-1532. doi: 10.1038/s41564-017-0013-6. Epub 2017 Sep 11.

Lu R., Schaefer C.M., Nesbitt N.M., Kuper J., Kisker C., Sampson N.S. (2017). Catabolism of the Cholesterol Side Chain in Mycobacterium tuberculosis ls Controlled by a Redox-Sensitive Thiol Switch. ACS Infect Dis. 2017 Sep 8;3(9):666-675. doi: 10.1021/acsinfecdis.7b00072. Epub 2017 Aug 16.

Kaiser S., Sauer F., Kisker C. (2017). The structural and functional characterization of human RecQ4 reveals insights into its helicase mechanism. Nat Commun. 2017 Jun 27;8:15907. doi: 10.1038/ncomms15907.

Fischer A., Harrison K.S., Ramirez Y., Auer D., Chowdhury S.R., Prusty B.K., Sauer F., Dimond Z., Kisker C., Scott Hefty P., Rudel T. (2017). Chlamydia trachomatis-containing yacuole serves as deubiquitination platform to stabilize Mcl-1 and to interfere with host defense. Elife. 2017 Mar 28;6. pii: e21465. doi: 10.7554/eLife.21465.

Neckles C., Eltschkner S., Cummings J.E., Hirschbeck M., Daryaee F., Bommineni G.R., Zhang Z., Spagnuolo L., Yu W., Davoodi S., Slayden R.A., Kisker C., Tonge P.J. (2017). Rationalizing the Binding Kinetics for the Inhibition of the Burkholderia pseudomallei Fabl1 Enoyl-ACP Reductase. (2017). Biochemistry. 2017 Apr 4;56(13):1865-1878. doi: 10.1021/acs.biochem.6b01048. Epub 2017 Mar 21.

Spagnuolo L.A., Eltschkner S., Yu W., Daryaee F., Davoodi S., Knudson S.E., Allen E.K., Merino J., Pschibul A., Moree B., Thivalapill N., Truglio J.J., Salafsky J., Slayden R.A., Kisker C., Tonge P.J. (2017). Evaluating the Contribution of Transition-State Destabilization to Changes in the Residence Time of Triazole-Based InhA Inhibitors. J Am Chem Soc. 2017 Mar 8;139(9):3417-3429. doi: 10.1021/jacs.6b11148. Epub 2017 Feb 22.

Jung L.A., Gebhardt A., Koelmel W., Ade C.P., Walz S., Kuper J., von Eyss B., Letschert S., Redel C., d'Artista L., Biankin A., Zender L., Sauer M., Wolf E., Evan G., Kisker C., Eilers M. (2017). OmoMYC blunts promoter invasion by oncogenic MYC to inhibit gene expression characteristic of MYC-dependent tumors. Oncogene. 2017 Apr 6;36(14):1911-1924. doi: 10.1038/onc.2016.354. Epub 2016 Oct 17.

Lorenz S (2017). Structural mechanisms of HECT-type ubiquitin ligases. Biol Chem 399(2):127-145

Sander B, Xu W, Eilers M, Popov N, Lorenz S (2017). A conformational switch regulates the ubiquitin ligase HUWE1. eLife 6:e21036

Maric, H. M.*, Hausrat, T. J., Neubert, F., Dalby, N.O., Doose S., Sauer M., Kneussel M., Strømgaard K. (2017), Gephyrin-Binding Peptides Visualize Post-Synaptic Sites and Modulate Neurotransmission, Nature Chemical Biology, 13, 153–160 (2017) doi: 10.1038/nchembio.2246 (*First authorship and shared corresponding authorship)

Münzer P, Walker-Allgaier B, Geue S, Langhauser F, Geuss E, Stegner D, Aurbach K, Semeniak D, Chatterjee M, Gonzalez Menendez I, Märklin M, Quintanilla-Martinez L, Salih HR, Litchfield DW, Buchou T, Kleinschnitz C, Lang F, Nieswandt B, Pleines I, Schulze H, Gawaz M, Borst O. CK2β regulates thrombopoiesis and Ca(2+)-triggered platelet activation in arterial thrombosis. Blood. 2017 Dec 21;130(25):2774-2785. doi: 10.1182/blood-2017-05-784413.

Haining EJ, Matthews AL, Noy PJ, Romanska HM, Harris HJ, Pike J, Morowski M, Gavin RL, Yang J, Milhiet PE, Berditchevski F, Nieswandt B, Poulter NS, Watson SP, Tomlinson MG. Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation. Platelets. 2017 Nov;28(7):629-642. doi: 10.1080/09537104.2016.1254175.

Haining EJ, Cherpokova D, Wolf K, Becker IC, Beck S, Eble JA, Stegner D, Watson SP, Nieswandt B. CLEC-2 contributes to hemostasis independently of classical hemITAM signaling in mice. Blood. 2017 Nov 16;130(20):2224-2228. doi: 10.1182/blood-2017-03-771907.

Manukjan G, Bösing H, Schmugge M, Strauß G, Schulze H. Impact of genetic variants on haematopoiesis in patients with thrombocytopenia absent radii (TAR) syndrome. Br J Haematol. 2017 Nov;179(4):606-617. doi: 10.1111/bjh.14913.

Beck S, Leitges M, Stegner D. Protein kinase Cι/λ is dispensable for platelet function in thrombosis and hemostasis in mice. Cell Signal. 2017 Oct;38:223-229.  doi: 10.1016/j.cellsig.2017.07.015.

Drube S, Grimlowski R, Deppermann C, Fröbel J, Kraft F, Andreas N, Stegner D, Dudeck J, Weber F, Rödiger M, Göpfert C, Drube J, Reich D, Nieswandt B, Dudeck A, Kamradt T. The Neurobeachin-like 2 Protein Regulates Mast Cell Homeostasis. J Immunol. 2017 Oct 15;199(8):2948-2957. doi: 10.4049/jimmunol.1700556.

Stritt S, Beck S, Becker IC, Vögtle T, Hakala M, Heinze KG, Du X, Bender M, Braun A, Lappalainen P, Nieswandt B. Twinfilin 2a regulates platelet reactivity and turnover in mice. Blood. 2017 Oct 12;130(15):1746-1756. doi: 10.1182/blood-2017-02-770768.

Schulze H, Schlagenhauf A, Manukjan G, Beham-Schmid C, Andres O, Klopocki E, König EM, Haidl H, Panzer S, Althaus K, Muntean WE, Schwinger W, Urban C, Greinacher A, Bakchoul T, Seidel MG. Recessive grey platelet-like syndrome with unaffected erythropoiesis in the absence of the splice isoform GFI1B-p37. Haematologica. 2017 Sep;102(9):e375-e378. doi: 10.3324/haematol.2017.167957.

Stegner D, van Eeuwijk JMM, Angay O, Gorelashvili MG, Semeniak D, Pinnecker J, Schmithausen P, Meyer I, Friedrich M, Dütting S, Brede C, Beilhack A, Schulze H, Nieswandt B, Heinze KG. Thrombopoiesis is spatially regulated by the bone marrow vasculature. Nat Commun. 2017 Jul 25;8(1):127. doi: 10.1038/s41467-017-00201-7.

Dütting S, Gaits-Iacovoni F, Stegner D, Popp M, Antkowiak A, van Eeuwijk JMM, Nurden P, Stritt S, Heib T, Aurbach K, Angay O, Cherpokova D, Heinz N, Baig AA, Gorelashvili MG, Gerner F, Heinze KG, Ware J, Krohne G, Ruggeri ZM, Nurden AT, Schulze H, Modlich U, Pleines I, Brakebusch C, Nieswandt B. A Cdc42/RhoA regulatory circuit downstream of glycoprotein Ib guides transendothelial platelet biogenesis. Nat Commun. 2017 Jun 15;8:15838. doi: 10.1038/ncomms15838.

Mammadova-Bach E, Mauler M, Braun A, Duerschmied D. Immuno-Thrombotic Effects of Platelet Serotonin. Chapter 12: Book Neuroscience "Serotonin - A Chemical Messenger Between All Types of Living Cells", 2017;10.5772/intechopen.69349.

Bender M, Stegner D, Nieswandt B. Model systems for platelet receptor shedding. Platelets. 2017 Jun;28(4):325-332. doi: 10.1080/09537104.2016.1195491.

Jamasbi J, Ayabe K, Goto S, Nieswandt B, Peter K, Siess W. Platelet receptors as therapeutic targets: Past, present and future. Thromb Haemost. 2017 Jun 28;117(7):1249-1257. doi: 10.1160/TH16-12 0911.

Andres O, Wiegering V, König EM, Schneider AL, Semeniak D, Stritt S, Klopocki E, Schulze H. A novel two-nucleotide deletion in HPS6 affects mepacrine uptake and platelet dense granule secretion in a family with Hermansky-Pudlak syndrome. Pediatr Blood Cancer. 2017 May;64(5). doi: 10.1002/pbc.26320.

Ta-Shma A, Zhang K, Salimova E, Zernecke A, Sieiro-Mosti D, Stegner D, Furtado M, Shaag A, Perles Z, Nieswandt B, Rein AJ, Rosenthal N, Neiman AM, Elpeleg O. Congenital valvular defects associated with deleterious mutations in the PLD1 gene. J Med Genet. 2017 Apr;54(4):278-286. doi: 10.1136/jmedgenet-2016-104259.

Deppermann C, Kraft P, Volz J, Schuhmann MK, Beck S, Wolf K, Stegner D, Stoll G, Nieswandt B. Platelet secretion is crucial to prevent bleeding in the ischemic brain but not in the inflamed skin or lung in mice. Blood. 2017 Mar 23;129(12):1702-1706. doi: 10.1182/blood-2016-12-750711.

Pierre S, Linke B, Suo J, Tarighi N, Del Turco D, Thomas D, Ferreiros N, Stegner D, Frölich S, Sisignano M, Meyer Dos Santos S, deBruin N, Nüsing RM, Deller T, Nieswandt B, Geisslinger G, Scholich K. GPVI and Thromboxane Receptor on Platelets Promote Proinflammatory Macrophage Phenotypes during Cutaneous Inflammation. J Invest Dermatol. 2017 Mar;137(3):686-695. doi: 10.1016/j.jid.2016.09.036.

Nagy M, Mastenbroek TG, Mattheij NJA, de Witt S, Clemetson KJ, Kirschner J, Schulz AS, Vraetz T, Speckmann C, Braun A, Cosemans JMEM, Zieger B, Heemskerk JWM. Variable impairment of platelet functions in patients with severe, genetically linked immune deficiencies. Haematologica. 2018 Mar;103(3):540-549. doi: 10.3324/ haematol.2017.176974.

Pleines I, Woods J, Chappaz S, Kew V, Foad N, Ballester-Beltrán J, Aurbach K,  Lincetto C, Lane RM, Schevzov G, Alexander WS, Hilton DJ, Astle WJ, Downes K, Nurden P, Westbury SK, Mumford AD, Obaji SG, Collins PW, Delerue F, Ittner LM, Bryce NS, Holliday M, Lucas CA, Hardeman EC, Ouwehand WH, Gunning PW, Turro E, Tijssen MR, Kile BT. Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia. J Clin Invest. 2017 Mar 1;127(3):814-829. doi: 10.1172/JCI86154.

Latorre-Rey LJ, Wintterle S, Dütting S, Kohlscheen S, Abel T, Schenk F, Wingert S, Rieger MA, Nieswandt B, Heinz N, Modlich U. Targeting expression to megakaryocytes and platelets by lineage-specific lentiviral vectors. J Thromb Haemost. 2017 Feb;15(2):341-355. doi: 10.1111/jth.13582.

Schuhmann MK, Guthmann J, Stoll G, Nieswandt B, Kraft P, Kleinschnitz C. Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke. J Neuroinflammation. 2017 Jan 21;14(1):18. doi: 10.1186/s12974-017-0792-y.

Vorlova S, Koch M, Manthey HD, Cochain C, Busch M, Chaudhari SM, Stegner D, Yepes M, Lorenz K, Nolte MW, Nieswandt B, Zernecke A. Coagulation factor XII induces pro-inflammatory cytokine responses in macrophages and promotes atherosclerosis in mice. Thromb Haemost. 2017 Jan 5;117(1):176-187. doi: 10.1160/TH16-06-0466.

Misra M., Kuhn M., Löbel M., An H., Statsyuk A.V., Sotriffer C., Schindelin H. (2017). Dissecting the Specificity of Adenosyl Sulfamate Inhibitors Targeting the Ubiquitin-Activating Enzyme. Structure. 2017 Jul 5;25(7):1120-1129.e3. doi: 10.1016/j.str.2017.05.001. Epub 2017 Jun 1.

Jank T, Belyi Y, Wirth C, Rospert S, Hu Z, Dengjel J, Tzivelekidis T, Andersen GR, Hunte C, Schlosser A, Aktories K. (2017) Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A. J Biol Chem 292:16014-16023.

Bach M, Lehmann A, Brünnert D, Vanselow JT, Hartung A, Bargou RC, Holzgrabe U, Schlosser A, Chatterjee M. (2017) Ugi Reaction-Derived α-Acyl Aminocarboxamides Bind to Phosphatidylinositol 3-Kinase-Related Kinases, Inhibit HSF1-Dependent Heat Shock Response, and Induce Apoptosis in Multiple Myeloma Cells. J Med Chem 60:4147-4160.

Uthe H, Vanselow JT, Schlosser A. Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae. (2017) Sci Rep. 7:43584.

Fazeli, G., Wehman, A.M. (2017). Safely removing cell debris with LC3-associated phagocytosis.Biol Cell. DOI: 10.1111/boc.201700028

Fazeli, G., Wehman, A.M. (2017). Rab GTPases mature the LC3-associated midbody phagosome.Comm Int Biol 10(2): e1297349. DOI: 10.1080/19420889.2017.1297349

Beer, K.B., Wehman A.M. (2017) Mechanisms and functions of extracellular vesicle release in vivo - What we can learn from flies and worms. Cell Adh Migr 11(2):135-150. Review

Thorn, A., Parkhurst, J.M., Emsley, P., Nicholls, R., Evans, G., Vollmar, M., Murshudov, G.N. (2017) AUSPEX: a graphical tool for X-ray diffraction data analysis, Acta Cryst D73 729-737.

Parkhurst, J.M., Thorn, A., Vollmar, M., Winter, G., Waterman, D.G., Fuentes-Montero, L., Gildea, R.J., Murshudov G.N., Evans, G. (2017) Background modelling of diffraction data in the presence of ice-rings, IUCrJ 4, 626-638.

Thorn, A. (2017) Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX, in Protein Crystallography, Methods in Molecular Biology (series), 357-376 co-editors A. Wlodawer, Z. Dauter, and M. Jaskolski, Springer. [invited book chapter]

 

 

2016

Biophysical characterization and activity of lymphostatin, a multifunctional virulence factor of attaching & effacing Escherichia coli. Cassady-Cain R. L., Blackburn E. A., Alsarraf H., Dedic E., Bease A. G., Böttcher B., Jorgensen R., Wear M. and Stevens M. P. (2016). J Biol Chem Jan 19; 291 5803-16. doi: 10.1074/jbc.M115.709600.

Cathelicidins Have Direct Antiviral Activity against Respiratory Syncytial Virus In Vitro and Protective Function In Vivo in Mice and Humans. Currie S. M., Gwyer Findlay E., McFarlane A. J., Fitch P. M., Böttcher B., Colegrave N., Paras A., Jozwik A., Chiu C., Schwarze J. and Davidson D. J. (2016). J Immunol Mar 15; 196 (6): 2699-710. doi: 10.4049/jimmunol.1502478.

Conrad M, Angeli JP, Vandenabeele P, Stockwell BR. Regulated necrosis: disease relevance and therapeutic opportunities. Nat Rev Drug Discov. 2016 May;15(5):348-66. doi: 10.1038/nrd.2015.6.

Gascón S, Murenu E, Masserdotti G, Ortega F, Russo GL, Petrik D, Deshpande A, Heinrich C, Karow M, Robertson SP, Schroeder T, Beckers J, Irmler M, Berndt C, Angeli JP, Conrad M, Berninger B, Götz M. Identification and Successful Negotiation of a Metabolic Checkpoint in Direct Neuronal Reprogramming. Cell Stem Cell. 2016 Mar 3;18(3):396-409. doi: 10.1016/j.stem.2015.12.003.

Daryaee F., Chang A., Schiebel J., Lu Y., Zhang Z., Kapilashrami K., Walker S.G., Kisker C., Sotriffer C.A., Fisher S.L., Tonge P.J. (2017). Correlating Drug-Target Kinetics and In vivo Pharmacodynamics: Long Residence Time Inhibitors of the Fabl Enoyl-ACP Reductase. Chem Sci. 2016 Sep 1;7(9):5945-5954. Epub 2016 May 25

Lorenzin F., Benary U., Baluapuri A., Walz S., Jung L.A., von Eyss B., Kisker C., Wolf J., Eilers M., Wolf E. (2016). Different promoter affinities account for specificity in MYC-dependent gene regulation.Elife. 2016 Jul 27;5. pii: e15161. doi: 10.7554/eLife.15161. 

Wirth, N., Gross, J., Roth, H.M., Buechner, C.N., Kisker, C., Tessmer, I. (2016) Conversation and Divergence in Nucleotide Excision Repair Lesion Recognition. (2016). J Biol Chem. Jul 12. pii: jbc.M116.739425.

Houten, B.V., Kuper, J., Kisker, C. (2016). Role of XPD in cellular functions: To TFIIH and beyond. DNA Repair (Amst). May 16. pii: S1568-7864(16)30099-4. doi: 10.1016/j.dnarep.2016.05.019.

Neckles,C., Pschibul, A., Lai, C.T., Hirschbeck, M., Kuper, J., Davoodi, S., Zou, J., Liu, N., Pan, P., Shah, S., Daryaee, F., Bommineni, G.R., Lai, C., Simmerling, C., Kisker, C., Tonge, P.J. Selectivity of Pyridone- and Diphenyl Ether-Based Inhibitors for the Yersinia pestis FabV Enoyl-ACP Reductase. Biochemistry. May 31;55(21):2992-3006. doi:10.1021/acs.biochem.5b01301. Epub 2016 May 17.

Rohleder, F., Huang, J., Xue, Y., Kuper, J., Round, A., Seidman, W., Wang, W., Kisker, C. (2016). FANCM interacts with PCNA to promote replication traverse of DNA interstrand crosslinks.Nucleic Acids Res. Pii: gwk037.

Lorenz S, Bhattacharyya M, Feiler C, Rape M, Kuriyan J (2016). Crystal structure of a Ube2S-ubiquitin conjugate. PLoS One 11(2):e0147550

Cheng J., Sahani S., Hausrat T. J., Yang J. W., Ji H., Schmarowski N., Endle H., Liu X., Li Y., Bottche R., Radyushkin K., Maric H. M., Hoerder-Suabedissen A., Molnar Z., Prouvot P. H., Trimbuch T., Ninnemann O., Huai J., Fan W., Visentin B., Sabbadini R., Strømgaard K., Stroh A., Luhmann H. J., Kneussel M., Nitsch R. Vogt J.  (2016). Precise Somatotopic Thalamocortical Axon Guidance Depends on LPA-Mediated PRG-2/Radixin Signaling. Neuron 92(1): 126-142. doi: 10.1016/j.neuron.2016.08.035

Kasaragod, V.B., Schindelin, H. (2016). Structural Framework for Metal Incorporation during Molybdenum Cofactor Biosynthesis. Structure. May 3;24(5):782-8. doi: 10.1016/j.str.2016.02.023. Epub Apr 21.

Hänzelmann, P.; Schindelin, H. (2016). Characterization of an Additional Binding Surface on the p97 N-Terminal Domain Involved in Bipartite Cofactor Interactions. Structure. 24(1): 140-7. doi: 10.1016/j.str.2015.10.026. Epub 2015 Dec 17.

Fazeli, G., Trinkwalder, M., Irmisch, L., Wehman, A.M. (2016). C. elegans midbodies are released, phagocytosed, and undergo LC3-dependent degradation independent of macroautophagy. J Cell Sci 129(20):3721-3731

Knott, G.J, Panjikar, S., Thorn, A., Fox, A.H., Conte, M.R., Lee, M., Bond, C.S. (2016) A crystallographic study of human NONO (p54nrb): Overcoming pathological problems with purification, data collection and non-crystallographic symmetry, Acta Cryst. D72, 761-769

 

 

2015

Conrad M, Friedmann Angeli JP. Glutathione peroxidase 4 (Gpx4) and ferroptosis: what's so special about it? Mol Cell Oncol. 2015 Jan 30;2(3):e995047. doi: 10.4161/23723556.2014.995047.

Pelz, J.P., Schindelin, H., van Pee, K., Kuper, J., Kisker, C., Diederichs, K., Fischer, U., Grimm, C. (2015). Crystrallizing the 6S and 8S spliceosomal assembly intermediates: a complex project. Acta Crystallogr D Biol Crystallogr. 2015 Oct;71(Pt 10):2040-53. doi: 10.1107/S1399004715014832. Epub 2015 Sep 26.

Koch, S.C., Kuper, J., Gasteiger, K.L., Simon, N., Strasser, R., Eisen, D., Geiger, S., Schneider, S., Kisker, C., Carell, T. (2015). Structural insights into the recognition of cisplatin and AAF-dG lesion by Rad14 (XPA).Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8272-7. doi: 10.1073/pnas.1508509112. Epub 2015 Jun 2.

Schiebel, J., Chang, A., Merget, B., Bommineni, G.R., Yu, W., Spagnuolo, L.A., Baxter, M.V., Tareilus, M., Tonge, P.J., Kisker, C., Sotriffer, C.A. (2015). An ordered water channel in Staphylococcus aureus FabI: unraveling the mechanism of substrate recognition and reduction. Biochemistry. 2015 Mar 17;54(10):1943-55. doi: 10.1021/bi5014358. Epub 2015 Mar 3.

Lorenz S, Deng T, Hantschel O, Superti-Furga G, Kuriyan J (2015). Crystal structure of an SH2-kinase construct of c-Abl and effect of the SH2 domain on kinase activity. Biochemical Journal, 468(2):283-91

Maric H. M., Kasaragod V. B., Haugaard-Kedström L., Hausrat T. J., Kneussel, M., Schindelin, H. and Strømgaard, K. (2015), Design and Synthesis of High-Affinity Dimeric Inhibitors Targeting the Interactions between Gephyrin and Inhibitory Neurotransmitter Receptors. Angewandte Chemie International Edition 2015, 54, p. 490–494. doi: 10.1002/anie.201409043 Selected as Hot Paper – Outstanding importance in a rapidly evolving field of high current interest

Hänzelmann, P.; Schindelin, H. (2016). Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97. Structure. 24(1):127-39. doi: 10.1016/j.str.2015.10.026. Epub 2015 Dec 17.

elto C.F., Heisler F.F., Kuper J., Sander B., Kneussel M., Schindelin H. (2015). The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Structure. 2015 Feb 3;23(2):364-73. doi: 10.1016/j.str.2014.11.016. Epub 2015 Jan 8.

ElBashir R, Vanselow JT, Kraus A, Janzen CJ, Siegel NT, Schlosser A. (2015) Fragment Ion Patchwork Quantification for Measuring Site-Specific Acetylation Degrees. Anal Chem 87:9939–9945.

Dufner A, Kisser A, Niendorf S, Basters A, Reissig S, Schönle A, Aichem A, Kurz T, Schlosser A, Yablonski D, Groettrup M, Buch T, Waisman A, Schamel WW, Prinz M, Knobeloch KP. (2015) The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells. Nat Immunol. 16:950–960. doi:10.1038/ni.3230.

Schmitt F, Vanselow, JT, Schlosser A, Kahnt J, Rössler W, Wegener C. (2015) Neuropeptidomics of the carpenter ant Camponotus floridanus. J. Proteome Res. 14:1504-1514.

 

 

2014

Friedmann Angeli JP, Schneider M, Proneth B, Tyurina YY, Tyurin VA, Hammond VJ, Herbach N, Aichler M, Walch A, Eggenhofer E, Basavarajappa D, Rådmark O, Kobayashi S, Seibt T, Beck H, Neff F, Esposito I, Wanke R, Förster H, Yefremova O, Heinrichmeyer M, Bornkamm GW, Geissler EK, Thomas SB, Stockwell BR, O'Donnell VB, Kagan VE, Schick JA, Conrad M. Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice. Nat Cell Biol. 2014 Dec;16(12):1180-91. doi: 10.1038/ncb3064.

Schaefer, C.M., Lu, R., Nesbitt, N.M., Schiebel, J., Sampson, N.S., and Kisker, C. (2015).  FadA5 a Thiolase from Mycobacterium tuberculosis: A Steroid-Binding Pocket Reveals the Potential for Drug Development against Tuberculosis. Structure 23(1):21-33. Doi: 10.1016/j.str2014.10.010. Epub 2014 Dec 4.

Calebiro, D., Hannawacker, A., Lyga, S., Bathon, K., Zabel, U., Ronchi, C., Beuschlein, F., Reincke, M., Lorenz, K., Alllolio, B., Kisker, C., Fassnacht, M., and Lohse, M.J. (2014). PKA catalytic subunit mutations in adrenocortical Cushing`s adenoma impair association with the regulatory subunit. Nat Commun 5:5680. doi: 10.1038/ncomms6680.

Khan, I., Suhasini, A.N., Banerjee, T., Sommers, J.A., Kaplan, D.L., Kuper, J., Kisker, C., and Brosh, R.M. Jr. (2014). Impact of age-associated cyclopurine lesions on DNA repair helicases. PLoS One 9(11): e113293. doi: 10.1371/journal.pone.0113293. eCollection 2014.

Kuper, J., Braun, C., Elias, A., Michels, G., Sauer, F., Schmitt, D.R., Poterszman, A., Egly, J.M., and Kisker, C. (2014). In TFIIH, XPD helicase is exclusively devoted to DNA repair. PLoS Biol 12(9): e1001954. doi:  10.1371/journal.pbio.1001954. eCollection 2014.

Di Dalmazi, G., Kisker, C., Calebiro, D., Mannelli, M., Canu, L., Arnaldi, G., Quinkler, M., Rayes, N., Tabarin, A., Laure Jullié, M., Mantero, F., Rubin, B., Waldmann, J., Bartsch, D.K., Pasquali, R., Lohse, M., Allolio, B., Fassnacht, M., Beuschlein, F., and Reincke, M. (2014). Novel somatic mutations in the catalytic subunit of the protein kinase A as a cause of adrenal Cushing`s syndrome: a European multicentric study. J Clin Endocrinol Metab. 99(10): E2093-100. doi 10.1210/jc.2014-2152. Epub 2014 Jul 24.

Maric, H. M.*, Kasaragod, V. B.*, Schindelin, H. (2014), Molecular basis of the alternative recruitment of GABAA versus glycine receptors through gephyrin. Nature Communications 2014, 5(5767). doi: 10.1038/ncomms6767 (*shared first authorship)

Maric, H. M., Kasaragod, V. B., Schindelin, H. (2014), Modulation of the Gephyrin-Glycine Receptor Affinity by Multivalency. ACS Chemical Biology 2014, 9(11), p. 2554–2562. doi: 10.1021/cb500303a

Soykan T., Schneeberger D., Tria G., Buechner C., Bader N., Svergun D., Tessmer I, Poulopoulos A, Papadopoulos T, Varoqueaux F, Schindelin H, Brose N. (2014). A conformational switch in collybistin determines the differentiation of inhibitory postsynapses. EMBO J. 2014 Sep 17;33(18):2113-33. doi: 10.15252/embj.201488143. Epub 2014 Jul 30.

Young JC, Clements A, Lang AE, Garnett JA, Munera D, Arbeloa A, Pearson J, Hartland EL, Matthews SJ, Mousnier A, Barry DJ, Way M, Schlosser A, Aktories K, Frankel G. (2014) The Escherichia coli effector EspJ blocks Src kinase activity via amidation and ADP ribosylation. Nat Commun 5:5887 | DOI: 10.1038/ncomms6887.

Deigan Warner, K.D., Chen, M.C., Song, W., Strack R.L., Thorn, A., Jaffrey S.R., Ferré-D’Amaré, A.R. (2014) Structural basis for activity of highly efficient RNA mimics of green fluorescent protein , Nature Structural & Molecular Biology 21, 658–663

 

 

2013

Lorenz S, Cantor AJ, Rape M, Kuriyan J (2013). Macromolecular juggling by ubiquitylation enzymes. BMC Biology 11: 65

Grimm C., Chari A., Pelz J.P., Kuper J., Kisker C., Diederichs K., Stark H., Schindelin H., Fischer U. (2013). Structural basis of assembly chaperone- mediated snRNP formation. Mol Cell. 2013 Feb 21;49(4):692-703. doi: 10.1016/j.molcel.2012.12.009. Epub 2013 Jan 17.

Thorn, A., Sheldrick G.M. (2013) Extending Molecular Replacement Solutions with SHELXE , Acta Cryst. D. 69, 2251-2256

 

 

2012

Tretter V., Mukherjee J., Maric H. M., Schindelin H., Sieghart W., Moss S. J. (2012), Gephyrin, the enigmatic organizer at GABAergic synapses. Frontiers in Cellular Neuroscience 2012. 6: p. 23. doi: 10.3389/fncel.2012.00023.

Sumara, G., Sumara, O., Kim, J., Karsenty, G. (2012) Gut-derived serotonin is a multifunctional determinant to fasting adaptation. Cell Metabolism, 16(5):588-600.

Cooper, R.I., Thorn, A., Watkin D.J. (2012) CRYSTALS enhancements: asymmetric restraints, J. Appl. Cryst. 45, 1057-1060

Thorn, A., Steinfeld, R., Ziegenbein, M., Grapp, M., Hsiao, H.H., Urlaub, H., Sheldrick, G.M., Gärtner, J., Krätzner, R. (2012) Structure and activity of the only human RNase T2, Nucleic Acids Res. 40, 8733-8742

Thorn, A., Dittrich, B., Sheldrick, G.M. (2012) Enhanced rigid-bond restraints, Acta Cryst. A68, 448-451

 

 

2011

Wickliffe KE, Lorenz S*, Wemmer DE, Kuriyan J, Rape M (2011). The mechanism of linkage-specific ubiquitin chain elongation by a single subunit E2. Cell 144(5): 769-781

Maric, H. M., Mukherjee J., Tretter, V., Moss, S. J., & Schindelin, H. (2011), Gephyrin-mediated γ-Aminobutyric Acid Type A and Glycine Receptor Clustering Relies on a Common Binding Site.The Journal of Biological Chemistry 2011, 286(49), p. 42105-42114. doi:10.1074/jbc.M111.303412

Mukherjee J., Kretschmannova K.*, Gouzer G.*, Maric H. M.*, Ramsden S., Tretter V., Harvey K., Davies P.A., Triller A., Schindelin H., Moss S.J. (2011), The residence time of GABAARs at inhibitory synapses is determined by direct binding of the receptor α1 subunit to gephyrin. The Journal of Neuroscience 2011, 31(41), p. 14677-87. doi:  10.1523/JNEUROSCI.2001-11.2011 (*shared second authorship.)

Tretter V., Kerschner B., Milenkovic I., Ramsden S. L., Ramerstorfer J., Saiepour L., Maric H.M., Moss S. J., Schindelin H., Harvey R. J., Sieghart W., Harvey K. (2011), Molecular basis of the GABAA α3 subunit interaction with the clustering protein gephyrin. The Journal of Biological Chemistry 2011, 286(43): p. 37702-11. doi: 10.1074/jbc.M111.291336

Hänzelmann P., Schindelin H. (2011). The structural and functional basis of the p97/valosin-containing protein (VCP)-interacting motif (VIM): mutually exclusive binding of cofactors to the N-terminal domain of p97. J Biol Chem. 2011 Nov 4;286(44):38679-90. doi: 10.1074/jbc.M111.274506. Epub 2011 Sep 13.

Hänzelmann P., Buchberger A., Schindelin H. (2011). Hierarchical binding of cofactors to the AAA ATPase p97. Structure. 2011 Jun 8;19(6):833-43. doi: 10.1016/j.str.2011.03.018.

Wehman, A.M., Poggioli, C., Schweinsberg, P., Grant, B.D., Nance, J. (2011). The phospholipid flippase TAT-5 inhibits the budding of extracellular vesicles in C. elegans embryos. Curr Biol 21(23):1951-1959.

Thorn, A., Sheldrick, G.M. (2011) ANODE: ANOmalous and heavy-atom DEnsity calculation, J. Appl. Cryst. 44, 1285-1287

 

 

2010

Tavcar, G., Sen, S.S., Azhakar, R., Thorn, A., Roesky, H.W. (2010) Facile syntheses of silylene nickel carbonyl complexes from Lewis base stabilized chlorosilylenes Inorg Chem. 49, 10199-10202

Vouffo, B., Dongo, E., Facey, P., Thorn, A., Sheldrick, G. M., Maier, A., Fiebig, H.H., Laatsch, H. (2010) Antiarol cinnamate and Africanoside, a cinnamoyl triterpene and a hydroperoxy-cardenolide from the stem bark of Antiaris africana, Planta Med. 76, 1717-1723

Fischer, A., Stern, D., Thorn, A., Abraham, S., Stalke, D., Klingebiel, U. (2010) From the Lithium-2-anilide-2-fluoro-1,3-diaza-2-sila-cyclopentene-GaCl3 Adduct to 1,4,6-Triaza-5-gallium-7-sila-cyclo-3-heptene - Experimental and Quantum-chemical Results Z. Anorg. Allg. Chem. 636 (2010), 1527-1532

 

 

2009

Sumara, G., Formentini, I., Collins, S., Sumara, I., Windak, R., Bodenmiller, B., Ramracheya, R., Caille, D., Jiang, H., Platt, K.A., Meda, P., Aebersold, R., Rorsman, P., Ricci, R. (2009) Regulation of PKD by the MAPK p38delta in insulin secretion and glucose homeostasis. Cell 136(2):235-48.

Vollmar, D., Thorn, A., Schuberth, I., Grond, S. (2009) A comprehensive view on 4-methyl-2-quinazolinamine, a new microbial alkaloid from Streptomyces of TCM plant origin, J. Antibiot. 62, 439-444

 

 

2008

Lee I., Schindelin H. (2008). Structural insights into E1-catalyzed ubiquitin activation and transfer to conjugating enzymes. Cell. 2008 Jul 25;134(2):268-78. doi: 10.1016/j.cell.2008.05.046.

Thorn, A., Egerer-Sieber, C., Jäger, C.M., Herl, V., Müller-Uri, F., Kreis, W., Muller Y.A. (2008) The crystal structure of 5β-Progesterone Reductase from Digitalis Lanata defines of a novel subfamily of short-chain dehydrogenases/reductases, J. Biol. Chem. 283, 17260-17269

Tampier, S., Müller, R., Thorn, A., Hübner, E., Burzlaff, N. (2008) Synthesis, Structure and Reactivity of Ruthenium Carboxylato and 2-Oxocarboxylato Complexes bearing the Bis(3,5-dimethylpyrazol-1-yl)acetato Ligand, Inorg Chem. 47 , 9624-9641

 

 

2006

Kim E.Y., Schrader N., Smolinsky B., Bedet C., Vannier C., Schwarz G., Schindelin H. Deciphering the structural framework of glycine receptor anchoring by gephyrin. EMBO J. 2006 Mar 22;25(6):1385-95. Epub 2006 Mar 2.

Tian G., Xiang S., Noiva R., Lennarz W.J., Schindelin H. (2006). The crystal structure of yeast protein disulfide isomerase suggests cooperativity between its active sites. Cell. 2006 Jan 13;124(1):61-73. Erratum in: Cell. 2006 Mar 10;124(5):1085-8.

 

 

2004

Hänzelmann P., Schindelin H. (2004). Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans. Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12870-5. Epub 2004 Aug 18.

 

 

2001

Lake M.W., Leimkühler S., Wuebbens M.M., Rajagopalan K.V., H. Schindelin. (2001). “The crystal structure of the MoeB-MoaD complex provides insights into the mechanism of ubiquitin activation”, Nature 414, 325-329.

Rudolph M.J., Wuebbens M.M., Rajagopalan K.V., Schindelin H. (2001). Crystal structure of molybdopterin synthase and its evolutionary relationship to ubiquitin-activation. Nature Struct. Biol. 8, 42-46.